Description

CRH is a 41-amino acid peptide derived by enzymatic cleavage from a 191-amino acid preprohormone and is located on 8q13. It is made not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes, and is expressed in very large amounts in the human placenta. As a placental clock, CRH controls the duration of pregnancy and that the timing of the rise in CRH may permit prediction of the onset of labor. Furthermore, CRH is the most proximal element of the HPA axis, and it acts as a central coordinator for neuroendocrine and behavioral responses to stress. In addition, placental secretion of CRH is a marker of the 'placental clock' that is active from an early stage in human pregnancy and determines the length of gestation and the timing of parturition and delivery.

Catalog Number

IW-PA1128

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide mapping at the N-terminal of human CRH, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Brain (hypothalamus), placenta, thymus.

Cellular Localization

Secreted

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Suitable.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Robinson, B. G.; Emanuel, R. L.; Frim, D. M.; Majzoub, J. A. : Glucocorticoid stimulates expression of corticotropin-releasing hormone gene in human placenta. Proc. Nat. Acad. Sci. 85: 5244-5248, 1988.

2. Inder, W. J.; Prickett, T. C. R.; Ellis, M. J.; Hull, L.; Reid, R.; Benny, P. S.; Livesey, J. H.; Donald, R. A. : The utility of plasma CRH as a predictor of preterm delivery. J. Clin. Endocr. Metab. 86: 5706-5710, 2001.

3. McLean, M.; Bisits, A.; Davies, J.; Woods, R.; Lowry, P.; Smith, R. : A placental clock controlling the length of human pregnancy. Nature Med. 1: 460-463, 1995.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human adenocarcinoma endometrium using CRH antibody.