CRH IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1128 9 ml $258 Yes

Description

CRH is a 41-amino acid peptide derived by enzymatic cleavage from a 191-amino acid preprohormone and is located on 8q13. It is made not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes, and is expressed in very large amounts in the human placenta. As a placental clock, CRH controls the duration of pregnancy and that the timing of the rise in CRH may permit prediction of the onset of labor. Furthermore, CRH is the most proximal element of the HPA axis, and it acts as a central coordinator for neuroendocrine and behavioral responses to stress. In addition, placental secretion of CRH is a marker of the 'placental clock' that is active from an early stage in human pregnancy and determines the length of gestation and the timing of parturition and delivery.

 

Catalog Number

 

IW-PA1128

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide mapping at the N-terminal of human CRH, identical to the related rat and mouse sequence.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Brain (hypothalamus), placenta, thymus.

 

Cellular Localization

 

Secreted

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Suitable.

ICC: Suitable.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Robinson, B. G.; Emanuel, R. L.; Frim, D. M.; Majzoub, J. A. : Glucocorticoid stimulates expression of corticotropin-releasing hormone gene in human placenta. Proc. Nat. Acad. Sci. 85: 5244-5248, 1988.

2. Inder, W. J.; Prickett, T. C. R.; Ellis, M. J.; Hull, L.; Reid, R.; Benny, P. S.; Livesey, J. H.; Donald, R. A. : The utility of plasma CRH as a predictor of preterm delivery. J. Clin. Endocr. Metab. 86: 5706-5710, 2001.

3. McLean, M.; Bisits, A.; Davies, J.; Woods, R.; Lowry, P.; Smith, R. : A placental clock controlling the length of human pregnancy. Nature Med. 1: 460-463, 1995.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded human adenocarcinoma endometrium using CRH antibody.

 

 

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