Description

Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

Catalog Number

IW-PA1113

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide mapping at the C-terminal of human Caspase-2, identical to the related rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, rat. Not tested in other species.

Positive Control

Spleen, intestine, testis, lymph node.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1.Bergeron, L.; Perez, G. I.; Macdonald, G.; Shi, L.; Sun, Y.; Jurisicova, A.; Varmuza, S.; Latham, K. E.; Flaws, J. A.; Salter, J. C. M.; Hara, H.; Moskowitz, M. A.; Li, E.; Greenberg, A.; Tilly, J. L.; Yuan, J. : Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 12: 1304-1314, 1998.

2.Tinel, A.; Tschopp, J. : The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. Science 304: 843-846, 2004.  

Images

Immunohistochemical analysis of paraffin-embedded sections. Rat spleen tissue using Caspase-2 antibody

Immunohistochemical analysis of paraffin-embedded sections. Rat intestinal cancer using Caspase-2 antibody

Immunohistochemical analysis of paraffin-embedded  human mammary cancer tissue sections using Caspase-2 antibody