Caspase-2 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1113 9 ml $258 Yes

Description

Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

 

Catalog Number

 

IW-PA1113

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide mapping at the C-terminal of human Caspase-2, identical to the related rat sequence.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, rat. Not tested in other species.

Positive Control

 

Spleen, intestine, testis, lymph node.

 

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1.Bergeron, L.; Perez, G. I.; Macdonald, G.; Shi, L.; Sun, Y.; Jurisicova, A.; Varmuza, S.; Latham, K. E.; Flaws, J. A.; Salter, J. C. M.; Hara, H.; Moskowitz, M. A.; Li, E.; Greenberg, A.; Tilly, J. L.; Yuan, J. : Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 12: 1304-1314, 1998.

2.Tinel, A.; Tschopp, J. : The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress. Science 304: 843-846, 2004.  

 

Images

 

Immunohistochemical analysis of paraffin-embedded sections. Rat spleen tissue using Caspase-2 antibody

 

Immunohistochemical analysis of paraffin-embedded sections. Rat intestinal cancer using Caspase-2 antibody

Immunohistochemical analysis of paraffin-embedded  human mammary cancer tissue sections using Caspase-2 antibody

 

 

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