Description

Collagen IV is comprised of a third chain (alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Good pasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.

Catalog Number

IW-MA1030

Quantity

9 ml

Host

Mouse

Clone

Col-30

Isotype

Mouse IgG1

Immunogen

Human collagen type IV

Purity

Purified by the goat anti-mouse IgG affinity chromatography.

Conjugate

Unconjugated

Species Reactivity

Human, rat. Not tested in other species.

Positive Control

Human mammary cancer, kidney, skin

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Formalin or acetone fixed frozen sections.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Kalluri, R.; Gattone, V. H., II; Noelken, M. E.; Hudson, B. G. : The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Proc. Nat. Acad. Sci. 91: 6201-6205, 1994.

2. Badenas, C.; Praga, M.; Tazon, B.; Heidet, L.; Arrondel, C.; Armengol, A.; Andres, A.; Morales. E.; Camacho, J. A.; Lens, X.; Davila, S.; Mila, M.; Antignac, C.; Darnell, A.; Torra, R. : Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria. J. Am. Soc. Nephrol. 13: 1248-1254, 2002.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer using Collagen Type IV antibody.