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Connexin 43 IHC Antibody
Ready To Use
| Catalog Number | Size | Price | Availability |
| IW-PA1026 | 9 ml | $258 | Yes |
Description
|
Connexins 43 (Cx43), also
called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a
member of the connexin gene family which abundantly
expressed in the heart and liver and was mapped to
6q21-q23.2. Connexin43, the major protein of gap junctions
in the heart, is targeted by several protein kinases that
regulate myocardial cell-cell coupling. Mutations in the
connexin43 gap-junction gene, which lead to abnormally
regulated cell-cell communication, are associated with
visceroatrial heterotaxia.
Cx43 must also play a critical role in the physiology
of hearing, presumably by participating in the recycling of
potassium to the cochlear endolymph. |
Catalog Number
|
IW-PA1026 |
Quantity
|
9 ml |
|
Host |
Rabbit |
|
Clone |
Polyclonal |
|
Isotype |
IgG |
|
Immunogen |
A peptide mapping at the C-terminus of
human Connexin 43, identical to the related rat and
mouse sequence. |
|
Purity |
Immunogen affinity purified |
|
Conjugate |
Unconjugated |
|
Species Reactivity |
Human, mouse, rat.
Not tested in other species. |
|
Positive Control |
Cardiac muscle |
|
Cellular Localization |
Membrane/cytoplasmic. |
|
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
|
Storage |
Store at 2-8
°C. Do not freeze. |
|
Applications |
IHC-P: Antigen retrieval is not required. Proteinase K digestion or heat induced epitope retrieval on formalin fixed paraffin sections may improve staining.
IHC-Fr: Not tested.
ICC: Suitable. |
|
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
|
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
|
References |
1. Britz-Cunningham, S. H.; Shah, M. M.; Zuppan, C. W.;
Fletcher, W. H. : Mutations of the connexin43 gap-junction
gene in patients with heart malformations and defects of
laterality. New Eng. J. Med. 332: 1323-1329, 1995.
2. Liu, X. Z.; Xia, X. J.; Adams, J.; Chen, Z. Y.; Welch,
K. O.; Tekin, M.; Ouyang, X. M.; Kristiansen, A.; Pandya,
A.; Balkany, T.; Arnos, K. S.; Nance, W. E. : Mutations in
GJA1 (connexin 43) are associated with non-syndromic
autosomal recessive deafness. Hum. Molec. Genet. 10:
2945-2951, 2001. |
|
Images |
Immunohistochemical analysis of formalin fixed paraffin-embedded
rat cardiac muscle using Connexin 43 antibody
|
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