Catalog Number | Size | Price | Availability |
IW-PA1027 | 9 ml | $258 | Yes |
Description
|
Cyclooxygenase 1 (COX1), also knows as Prostaglandin-endoperoxide
synthase (PTGS1), is the key enzyme in prostaglandin
biosynthesis. The gene was approximately 40 kb long, with 11
protein-coding exons. There were 599 amino acid residues
with a calculated molecular mass of approximately 68 kD. By
analysis of a human/hamster somatic hybrid DNA panel,
Funk et al. (1991) demonstrated that the PTGS1 gene maps to
chromosome 9.
Human prostaglandin endoperoxide synthase exhibited 91%
amino acid identity with the sheep enzyme.
Prostaglandin
synthase 1 gene disruption in mice reduces arachidonic
acid-induced inflammation and indomethacin-induced gastric
ulceration. |
Catalog Number
|
IW-PA1027 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide corresponding to a sequence near the
N-terminal of human PTGS1(COX1), different to the related
rat and mouse sequence by two amino acids. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Human, mouse, rat.
Not tested in other species. |
Positive Control |
Spleen |
Cellular Localization |
Cytoplasmic |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1. Yokoyama, C.; Tanabe, T. :
Cloning of human gene encoding prostaglandin endoperoxide synthase and
primary structure of the enzyme. Biochem. Biophys.
Res. Commun. 165: 888-894, 1989.
2. Langenbach, R.; Morham, S. G.;
Tiano, H. F.; Loftin, C. D.; Ghanayem, B. I.; Chulada, P.
C.; Mahler, J. F.; Lee, C. A.; Goulding, E. H.; Kluckman, K.
D.; Kim, H. S.; Smithies, O. :
Prostaglandin
synthase 1 gene disruption in mice reduces arachidonic
acid-induced inflammation and indomethacin-induced gastric
ulceration. Cell 83: 483-492, 1995.
|
Images |
Immunohistochemical analysis of formalin fixed paraffin-embedded
rat spleen tissue using COX1 antibody |
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