COX1 IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1027 9 ml $258 Yes

Description

Cyclooxygenase 1 (COX1), also knows as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9.  Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

 

Catalog Number

 

IW-PA1027

Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A synthetic peptide corresponding to a sequence near the N-terminal of human PTGS1(COX1), different to the related rat and mouse sequence by two amino acids.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Spleen

 

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Yokoyama, C.; Tanabe, T. : Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem. Biophys. Res. Commun. 165: 888-894, 1989.

2. Langenbach, R.; Morham, S. G.; Tiano, H. F.; Loftin, C. D.; Ghanayem, B. I.; Chulada, P. C.; Mahler, J. F.; Lee, C. A.; Goulding, E. H.; Kluckman, K. D.; Kim, H. S.; Smithies, O. : Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell 83: 483-492, 1995.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded rat spleen tissue using COX1 antibody. Staining COX1 in cytoplasm. DAB chromogenic reaction.

 

 

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