Catalog Number | Size | Price | Availability |
IW-PA1211 | 9 ml | $258 | Yes |
Description
|
Cyclooxygenase (Cox) is the key enzyme in conversion of
arachidonic acid to PGs, and two isoforms, Cox-1 and Cox-2,
have been identified. Cox-2 gene encodes an
inducible prostaglandin synthase enzyme that is
overexpressed in adenocarcinomas and other tumors. Deletion
of the murine Cox-2 gene in Min mice reduced the incidence
of intestinal tumors, suggesting that it is required for
tumorigenesis. This gene is localized to sites associated
with retinal blood vessels, and plays an important role in
blood vessel formation in the retina. And the
glucocorticoid receptor suppression of COX-2 is also crucial
for curtailing lethal immune activation, and suggest new
therapeutic approaches for regulation of T-cell-mediated
inflammatory diseases.
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Catalog Number
|
IW-PA1211 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
Rabbit IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence at the
N-terminal of human COX2, different to the related rat
sequence by two amino acids.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
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Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Human mammary cancer
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Cellular Localization
|
Cytoplasmic
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Form
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Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
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Applications
|
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin embedded tissue sections.
IHC-Fr: Not tested.
ICC: Not tested.
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Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
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References
|
1. Salmenkivi, K.; Haglund, C.;
Ristimaki, A.; Arola, J.; Heikkila, P. :
Increased
expression of cyclooxygenase
2. Liu, C. H.; Chang, S.-H.;
Narko, K.; Trifan, O. C.; Wu, M.-T.; Smith, E.; Haudenschild,
C.; Lane, T. F.; Hla, T. :
Overexpression of
cyclooxygenase-2 is sufficient to induce tumorigenesis in
transgenic mice. J. Biol. Chem. 276:
18563-18569, 2001.
3. Wilkinson-Berka, J. L.; Alousis, N. S.; Kelly, D. J.;
Gilbert, R. E. :
COX-2 inhibition and retinal angiogenesis in a mouse model
of retinopathy of prematurity. Invest. Ophthal.
Vis. Sci. 44: 974-979, 2003.
4. Brewer, J. A.; Khor, B.; Vogt, S. K.; Muglia, L. M.;
Fujiwara, H.; Haegele, K. E.; Sleckman, B. P.; Muglia, L. J.
: T-cell
glucocorticoid receptor is required to suppress
COX-2-mediated lethal immune activation. Nature
Med. 9: 1318-1322, 2003.
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Images
|
Immunohistochemical analysis of formalin fixed paraffin-embedded
human mammary cancer using COX2 antibody. Staining COX2 in
cytoplasm. DAB chromogenic reaction.
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