Description

Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.

Catalog Number

IW-MA1036

Quantity

9 ml

Host

Mouse

Clone

DES-82

Isotype

Mouse IgG1

Immunogen

Desmin from pig stomach.

Purity

Purified by the goat anti-mouse IgG affinity chromatography

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Skeletal muscle, cardiac tissue.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Tidball, J. G. : Desmin at myotendinous junctions. Exp. Cell Res. 199: 206-212, 1992.

2. Viegas-Pequignot, E.; Lin, L. Z.; Dutrillaux, B.; Apiou, F.; Paulin, D. : Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization. Hum. Genet. 83: 33-36, 1989.

3. Li, D.; Tapscoft, T.; Gonzalez, O.; Burch, P. E.; Quinones, M. A.; Zoghbi, W. A.; Hill, R.; Bachinski, L. L.; Mann, D. L.; Roberts, R. : Desmin mutation responsible for idiopathic dilated cardiomyopathy. Circulation 100: 461-464, 1999.

Images

Immunohistochemical staining of formalin fixed paraffin-embedded rat cardiac muscle using Desmin antibody. DAB chromogen substrate. 

Immunohistochemical staining of formalin fixed paraffin-embedded human cardiac muscle using Desmin antibody. DAB chromogen substrate.