Description

Elastin is a major structural protein of tissues such as aorta, which must expand rapidly and recover completely. Elastin chains are cross-linked together into an extensible 3D network. It is a molecular determinant of late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Defects in elastin are a cause of autosomal dominant cutis laxa.

Catalog Number

IW-MA1038

Quantity

9 ml

Host

Mouse

Clone

IL-4

Isotype

Mouse IgG1

Immunogen

Bovine α-elastin.

Purity

Purified by the goat anti-mouse IgG affinity chromatography

Conjugate

Unconjugated

Species Reactivity

Human, dog, guinea pig. Not tested in other species.

Positive Control

Skin, kidney, small intestine.

Cellular Localization

Extracellular

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Proteolytic Induced Epitope Retrieval (PIER) (Proteinase K) required on formalin fixed paraffin embedded tissue sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Rosenbloom, J. : Personal Communication. Philadelphia, Pa., 4/1984.

2. Urban, Z.; Riazi, S.; Seidl, T. L.; Katahira, J.; Smoot, L. B.; Chitayat, D.; Boyd, C. D.; Hinek, A. : Connection between elastin haploinsufficiency and increased cell proliferation in patients with supravalvular aortic stenosis and Williams-Beuren syndrome. Am. J. Hum. Genet. 71: 30-44, 2002.

3. Uitto, J.; Christiano, A. M.; Kahari, V.-M.; Bashir, M. M.; Rosenbloom, J. : Molecular biology and pathology of human elastin. Biochem. Soc. Trans. 19: 824-829, 1991.

Images

Immunohistochemical analysis of formalin fixed paraffin embedded human skin section using Elastin antibody showing elastic fiber staining.