Catalog Number | Size | Price | Availability |
IW-PA1101 | 9 ml | $258 | Yes |
Description
|
FAS Ligand (FASL) is a 40 kDa type II membrane protein
belonging to the tumor necrosis factor family, which induces
apoptosis by binding to its receptor, Fas. The human FasL
gene consists of approximately 8.0 kb and is split into four
exons. This gene consists of 281 amino acids with a
calculated M(r) of 31,759 and was mapped on chromosome 1q23.
It has an identity of 76.9% at the amino acid sequence level
with mouse FasL. The FAS and FASL system plays a key role in
regulating apoptotic cell death and corruption of this
signalling pathway has been shown to participate in immune
escape and tumorigenesis. FAS and FASL triggered apoptosis
pathway plays an important role in human carcinogenesis.
This system may also play a role in modulating
the genetic susceptibility of mouse strains to
develop T-cell lymphoblastic lymphomas.
|
Catalog Number
|
IW-PA1101 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
Rabbit IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence mapping at
the C-terminal of human FAS-L, different to the related
mouse sequence by three amino acids .
|
Purity
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Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
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Human intestinal cancer. |
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution. No further dilution needed. Serum blocking
step should be omitted.
|
Storage
|
Store at 2-8
°C. Do not freeze.
|
Applications
|
IHC-P: Formalin fixed paraffin embedded tissue sections.
IHC-Fr: Not tested.
ICC: Suitable.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1.
Takahashi, T.; Tanaka, M.; Inazawa,
J.; Abe, T.; Suda, T.; Nagata, S. :
Human Fas ligand:
gene structure, chromosomal location and species
specificity. Int. Immun. 6: 1567-1574, 1994.
2.
Zhang, X.; Miao, X.; Sun, T.; Tan,
W.; Qu, S.; Xiong, P.; Zhou, Y.; Lin, D. :
Functional
polymorphisms in cell death pathway genes FAS and FASL
contribute to the risk of lung cancer. J. Med.
Genet. 42: 479-484, 2005.
3.
Villa-Morales, M.; Santos, J.; Perez-Gomez, E.; Quintanilla,
M.; Fernandez-Piqueras, J. :
A role for the Fas/FasL
system in modulating genetic susceptibility to T-cell
lymphoblastic lymphomas. Cancer Res. 67:
5107-5116, 2007.
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Images
|
Immunohistochemical staining of formalin fixed paraffin-embedded human intestinal cancer using Fas-L antibody showing cytoplasmic staining.
|
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