Description

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Catalog Number

IW-PA1032

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A peptide mapping near the N-terminal of human FGF2, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human mammary cancer

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Doniach, T. : Basic FGF as an inducer of anteroposterior neural pattern. Cell 83: 1067-1070, 1995

2. Eckenstein, F. P. : Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480, 1994.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer tissue using FGF2 antibody. Staining FGF2 in cytoplasm. DAB chromogenic reaction.