Description

Fibroblast growth factor 4 (FGF4) also knows as Heparin Secretary Transforming (HSTF1). HST1, for which the designation HSTF1 was proposed for human gene nomenclature, is a heparin-binding growth factor with significant homology to human fibroblast growth factors and the mouse Int-2 protein. By in situ hybridization, Adelaide et al. (1988) mapped the HST gene to chromosome 11q13. The HST1 protein is a heparin-binding growth factor with significant homology with human fibroblast growth factors and the mouse Int-2 protein.

Catalog Number

IW-PA1033

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of the human FGF4, identical to the related mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human lung cancer

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Huebner, K.; Ferrari, A. C.; Delli Bovi, P.; Croce, C. M.; Basilico, C. : The FGF-related oncogene, K-FGF, maps to human chromosome region 11q13, possibly near int-2. Oncogene Res. 3: 263-270, 1988.

2. Adelaide, J.; Mattei, M.-G.; Marics, I.; Raybaud, F.; Planche, J.; De Lapeyriere, O.; Birnbaum, D. : Chromosomal localization of the hst oncogene and its co-amplification with the int.2 oncogene in a human melanoma. Oncogene 2: 413-416, 1988.

3. Yoshida, T.; Tsutsumi, M.; Sakamoto, H.; Miyagawa, K.; Teshima, S.; Sugimura, T.; Terada, M. : Expression of the HST1 oncogene in human germ cell tumors. Biochem. Biophys. Res. Commun. 155: 1324-1329, 1988.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human lung cancer section using FGF4 antibody showing cytoplasmic staining.