Catalog Number | Size | Price | Availability |
IW-PA1036 | 9 ml | $258 | Yes |
Description
|
Glutamic acid decarboxylase (GAD) catalyses the conversion
of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric
acid (GABA). Two forms of human GAD, GAD65 and GAD67, are
encoded by two separate genes.
Human GAD65 cDNA encodes a Mr 65,000 polypeptide,
with 585 amino acid residues, whereas human GAD67 encodes a
Mr 67,000 polypeptide, with 594 amino acid residues.
GAD67 gene consists of 16 exons, spread over more
than 45 kb of genomic DNA. The GAD67 gene contains an
additional exon (exon 0) that together with part of exon 1,
specifies the 5' untranslated region of GAD67 mRNA.
Human GAD67 shows 65% identity to GAD65 and is
located in 2q31. GAD67 may play a role in the stiff man
syndrome. Deficiency in this enzyme has been shown to lead
to pyridoxine dependency with seizures.
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Catalog Number
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IW-PA1036 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
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Immunogen
|
A peptide mapping very near
the N-terminal end
of GAD67
of human origin, identical to the related mouse sequence.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
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Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Brain
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Cellular Localization
|
Cytoplasmic
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Form
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Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Kelly, C. D.; Edwards, Y.; Johnstone, A. P.; Harfst, E.;
Nogradi, A.; Nussey, S. S.; Povey, S.; Carter, N. D. :
Nucleotide sequence and chromosomal assignment of a cDNA
encoding the large isoform of human glutamate decarboxylase.
Ann. Hum. Genet. 56: 255-265, 1992.
2. Bu, D.-F.; Erlander, M. G.; Hitz, B. C.; Tillakaratne, N.
J. K.; Kaufman, D. L.; Wagner-McPherson, C. B.; Evans, G.
A.; Tobin, A. J. : Two human glutamate decarboxylases,
65-kDa GAD and 67-kDa GAD, are each encoded by a single
gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992.
3. Bu, D.-F.; Tobin, A. J. : The exon-intron organization of
the genes (GAD1 and GAD2) encoding two human glutamate
decarboxylases (GAD-67 and GAD-65) suggests that they derive
from a common ancestral GAD. Genomics 21: 222-228, 1994.
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Images
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Immunohistochemical analysis of formalin fixed paraffin-embedded rat brain tissue section using GAD67 antibody showing cytoplasmic staining.
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