Glial fibrillary acidic protein(GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

IW-MA1045

9 ml

Mouse

GA-8

Mouse IgG1

GFAP from pig spinal cord.

Purified by the goat anti-mouse IgG affinity chromatography.

Unconjugated

Human, mouse, rat. Not tested in other species.

Brain

Cytoplasmic

Ready to use solution in PBS based antibody dilution buffer containing 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Store at 2-8 °C. Do not freeze.

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Formalin fixed or acetone fixed frozen sections.

ICC: Not tested.

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001.

2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.

Immunohistochemical staining of formalin fixed paraffin-embedded rat brain using GFAP antibody. DAB chromogen substrate.