Description

Glucocorticoid receptor (GR) maps to the distal long arm of chromosome 5. The human glucocorticoid receptor (hGR) gene contains a total of 10 exons and has a minimum size of 80 kilobases. The identification of complementary DNAs encoding the human glucocorticoid receptor (hGR) predicts two protein forms (alpha and beta; 777 and 742 amino acids long, respectively) which differ at their carboxy termini and both forms of the receptor are related, with respect to their domain structure, to the v-erb-A oncogene product of avian  erythroblastosis virus (AEV), which suggests that steroid receptor genes and the c-erb-A proto-oncogene are derived from a common primordial regulatory gene. Transcriptional regulation by the glucocorticoid receptor (GR) is mediated by hormone binding, receptor dimerization, and coactivator recruitment.

Catalog Number

IW-PA1107

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of

human GR, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human mammary cancer

Cellular Localization

Nuclear

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Suitable.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Encio, I. J.; Detera-Wadleigh, S. D. : The genomic structure of the human glucocorticoid receptor. J. Biol. Chem. 266: 7182-7188, 1991.

2. Weinberger, C.; Hollenberg, S. M.; Rosenfeld, M. G.; Evans, R. M. : Domain structure of human glucocorticoid receptor and its relationship to the v-erb-A oncogene product. Nature 318: 670-672, 1985.

3. Bledsoe, R. K.; Montana, V. G.; Stanley, T. B.; Delves, C. J.; Apolito, C. J.; McKee, D. D.; Consler, T. G.; Parks, D. J.; Stewart, E. L.; Willson, T. M.; Lambert, M. H.; Moore, J. T.; Pearce, K. H.; Xu, H. E.: Crystal structure of the glucocorticoid receptor ligand binding domain reveals a novel mode of receptor dimerization and coactivator recognition. Cell 110: 93-105, 2002.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer tissue section using GR antibody showing nuclear staining.