Catalog Number | Size | Price | Availability |
IW-PA1107 | 9 ml | $258 | Yes |
Description
|
Glucocorticoid receptor (GR) maps to the distal long arm of
chromosome 5. The human glucocorticoid receptor (hGR) gene
contains a total of 10 exons and has a minimum size of 80
kilobases. The identification of complementary DNAs encoding
the human glucocorticoid receptor (hGR) predicts two protein
forms (alpha and beta; 777 and 742 amino acids long,
respectively) which differ at their carboxy termini and both
forms of the receptor are related, with respect to their
domain structure, to the v-erb-A
oncogene product of avian erythroblastosis virus (AEV),
which suggests that steroid receptor genes and the c-erb-A
proto-oncogene are derived from a common primordial
regulatory gene. Transcriptional regulation by the
glucocorticoid receptor (GR) is mediated by hormone binding,
receptor dimerization, and coactivator recruitment
|
Catalog Number
|
IW-PA1107 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
Rabbit IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence at the
C-terminal of
human GR, identical to the related rat and mouse sequence.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not
tested in other species. |
Positive Control
|
Human mammary cancer |
Cellular Localization
|
Nuclear
|
Form
|
Ready to use solution. No further dilution needed. Serum blocking
step should be omitted.
|
Storage
|
Store at 2-8
°C. Do not freeze.
|
Applications
|
IHC-P: Formalin fixed paraffin embedded tissue sections.
IHC-Fr: Suitable.
ICC: Not tested.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Encio, I. J.; Detera-Wadleigh, S. D. : The genomic
structure of the human glucocorticoid receptor. J. Biol.
Chem. 266: 7182-7188, 1991.
2. Weinberger, C.; Hollenberg, S. M.; Rosenfeld, M. G.;
Evans, R. M. : Domain structure of human glucocorticoid
receptor and its relationship to the v-erb-A oncogene
product. Nature 318: 670-672, 1985.
3. Bledsoe, R. K.; Montana, V. G.; Stanley, T. B.; Delves,
C. J.; Apolito, C. J.; McKee, D. D.; Consler, T. G.; Parks,
D. J.; Stewart, E. L.; Willson, T. M.; Lambert, M. H.;
Moore, J. T.; Pearce, K. H.; Xu, H. E.: Crystal structure of
the glucocorticoid receptor ligand binding domain reveals a
novel mode of receptor dimerization and coactivator
recognition. Cell 110: 93-105, 2002.
|
Images
|
Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer tissue section using GR antibody showing nuclear staining.
|
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