Catalog Number | Size | Price | Availability |
IW-PA1041 | 9 ml | $258 | Yes |
Description
|
HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is
a transcription factor that mediates cellular and systemic
homeostatic responses to reduced O2 availability in mammals,
including angiogenesis, erythropoiesis and glycolysis.
This gene was mapped to 14q21-q24. HIF-1α
transactivate genes required for energy metabolism and
tissue perfusion and is necessary for embryonic development
and tumor explant growth. HIF-1alpha is over expressed
during carcinogenesis, myocardial infarction and wound
healing. It is
crucial for the cellular response to hypoxia and is
frequently over expressed in human cancers, resulting in the
activation of genes essential for cell survival.
HIF-1α regulates the survival and function in the
inflammatory microenvironment directly. It is a
transcription factor that plays a pivotal role in cellular
adaptation to changes in oxygen availability. |
Catalog Number
|
IW-PA1041 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide corresponding to a sequence mapping at
the C-terminal of human HIF-1α, identical to the related rat
and mouse sequence. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Human,
mouse,
rat.
Not tested in other species. |
Positive Control |
Skin, hypoxia induced tissues,
mammary cancer tissues |
Cellular Localization |
Cytoplasmic in
normoxia, nuclear in hypoxia. |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval is required for
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1. Sutter, C. H.; Laughner, E.; Semenza, G. L. :
Hypoxia-inducible factor 1-alpha protein expression is
controlled by oxygen-regulated ubiquitination that is
disrupted by deletions and missense mutations. Proc. Nat.
Acad. Sci. 97: 4748-4753, 2000.
2. Elson, D. A.; Thurston, G.; Huang, L. E.; Ginzinger, D.
G.; McDonald, D. M.; Johnson, R. S.; Arbeit, J. M. :
Induction of hypervascularity without leakage or
inflammation in transgenic mice overexpressing
hypoxia-inducible factor-1-alpha. Genes Dev. 15: 2520-2532,
2001.
3. Koshiji, M.; To, K. K.-W.; Hammer, S.; Kumamoto, K.;
Harris, A. L.; Modrich, P.; Huang, L. E. : HIF-1-alpha
induces genetic instability by transcriptionally
downregulating MutS-alpha expression. Molec. Cell 17:
793-803, 2005.
4. Ivan, M.; Kondo, K.; Yang, H.; Kim, W.; Valiando, J.;
Ohh, M.; Salic, A.; Asara, J. M.; Lane, W. S.; Kaelin, W.
G., Jr. : HIF-alpha targeted for VHL-mediated destruction by
proline hydroxylation: implications for O(2) sensing.
Science 292: 464-468, 2001. |
Images |
Immunohistochemical analysis of formalin fixed paraffin-embedded
human mammary cancer section using HIF-1α antibody |
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