Catalog Number | Size | Price | Availability |
IW-PA1129 | 9 ml | $258 | Yes |
Description
|
HIF-2 alpha is also designated EPAS1 whose gene is mapped
to
2p21-p16. The predicted mouse protein is 88%
identical to human EPAS1. The human EPAS1 gene contains 15
exons and spans at least 120 kb. The positions of the
introns within the genomic region encoding the N-terminal
bHLH-PAS domains of EPAS1 and AHR are similar, suggesting
that the 5-prime ends of the 2 genes may have arisen from a
gene duplication event1. Moreover, the predicted protein
shares 48% sequence identity with HIF1-alpha, a bHLH-PAS
transcription factor that induces EPO gene expression in
cultured cells in response to hypoxia. Like HIF1A, EPAS1
binds to and activates transcription from the HIF1A response
element derived from the 3-prime flanking region of the EPO
gene. EPAS1 is predominantly expressed in highly
vascularized tissues of adult humans and in endothelial
cells of the mouse adult and embryo. Furthermore, EPAS1 may
represent an important regulator of vascularization, perhaps
involving the regulation of endothelial cell gene expression
in response to hypoxia2. HIF2A is expressed at relatively
higher levels in villus sections of placenta and in lung
samples compared with other tissues examined3. In addition,
The variation in EPAS1 influences the relative contribution
of aerobic and anaerobic metabolism and hence the maximum
sustainable metabolic power for a given event duration4. |
Catalog Number
|
IW-PA1129 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide mapping at the amino acids 202-240 of rat HIF-2α. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Mouse, rat.
Not tested in other species. |
Positive Control |
Rat lung, small intestine. |
Cellular Localization |
Nuclear |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Formalin fixed or acetone fixed frozen sections.
ICC: Suitable. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1. Tian, H.; McKnight, S. L.; Russell, D. W. : Endothelial
PAS domain protein 1 (EPAS1), a transcription factor
selectively expressed in endothelial cells. Genes Dev. 11:
72-82, 1997.
2. Tian, H.; McKnight, S. L.; Russell, D. W. : Endothelial
PAS domain protein 1 (EPAS1), a transcription factor
selectively expressed in endothelial cells. Genes Dev. 11:
72-82, 1997.
3. Sood, R.; Zehnder, J. L.; Druzin, M. L.; Brown, P. O. :
Gene expression patterns in human placenta. Proc. Nat. Acad.
Sci. 103: 5478-5483, 2006.
4. Henderson, J.; Withford-Cave, J. M.; Duffy, D. L.; Cole,
S. J.; Sawyer, N. A.; Gulbin, J. P.; Hahn, A.; Trent, R. J.;
Yu, B. : The EPAS1 gene influences the aerobic-anaerobic
contribution in elite endurance athletes. Hum. Genet. 118:
416-423, 2005. |
Images |
Immunohistochemical analysis of frozen sections. Rat lung tissue using HIF-2α antibody
Immunohistochemical analysis of paraffin-embedded sections. Rat small intestine using HIF-2α antibody |
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