Catalog Number | Size | Price | Availability |
IW-PA1106 | 9 ml | $258 | Yes |
Description
|
HSP60 is a member of the
chaperonin class of protein factors, which include the
Escherichia coli groEL protein and the Rubisco
subunit-binding protein of chloroplasts. It acts as a
costimulator of human regulatory CD4-positive/CD25 -positive
T cells, which inhibit lymphoproliferation and IFNG and TNF
secretion by CD4-positive and CD8-positive T cells. HSP60
enhances Treg activity via TLR2, leading to activation of an
intracellular signaling cascade that included p38, as well
as inhibition of ERK phosphorylation. Suppression of target
T cells is mediated by both cell-to-cell contact and by
secretion of TGFB and IL10, and it leads to downregulation
of ERK, NFKB, and TBET expression. The self-molecule HSP60
can downregulate adaptive immune responses by upregulating
Tregs through TLR2 signaling.
|
Catalog Number
|
IW-PA1106 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A synthetic peptide
mapping at the middle region of human HSP60,
identical to the related rat and mouse sequence.
|
Purity
|
Immunogen affinity
purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Human intestinal cancer
|
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use
solution. No further dilution needed. Serum blocking step
should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Heat induced
epitope retrieval is required on formalin fixed paraffin
sections.
IHC-Fr: Suitable.
ICC: Suitable.
|
Limitations
|
This product is intended
for Research Use Only. Interpretation of the test
results is solely the responsibility of the user.
|
Precautions
|
Users should follow
general laboratory precautions when handling this product.
Wear personal protective equipment to avoid contact with
skin and eyes.
|
References
|
1.
Cheng, M. Y.;
Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.;
Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A.
L. : Mitochondrial
heat-shock protein hsp60 is essential for assembly of
proteins imported into yeast mitochondria.
Nature 337: 620-625, 1989.
2.
Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider,
O.; Cohen, I. R. :
Heat shock protein 60 enhances CD4+CD25+ regulatory T cell
function via innate TLR2 signaling. J. Clin.
Invest. 116: 2022-2032, 2006.
|
Images
|
Immunohistochemical
analysis of formalin fixed paraffin-embedded human intestinal cancer
using HSP60 antibody showing cytoplasmic staining.
|
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