Description

Interferon-induced Transmembrane Protein 1 (IFITM1), also called Interferon-induced Protein 17 (IFI17). IFITM1 activity is required for primordial germ cells (PGCs) transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. It is mapped to Chr.11 and belongs to the family of interferon-induced transmembrane proteins (Ifitm/mil/fragilis), which encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on their pattern of induction in interferon-sensitive and -resistant cell lines and the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth.

Catalog Number

IW-PA1112

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human IFITM1, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human. Not tested in other species.

Positive Control

Human intestine cancer, mammary cancer.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin embedded tissue sections.

IHC-Fr: Suitable.

ICC: Not tested

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Tanaka, S. S.; Yamaguchi, Y. L.; Tsoi, B.; Lickert, H.; Tam, P. P. L. : IFITM/Mil/Fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion. Dev. Cell 9: 745-756, 2005.

2. Deblandre, G. A.; Marinx, O. P.; Evans. S. S.; Majjaj, S.; Leo, O.; Caput, D.; Huez, G. A.; Wathelet, M. G. : Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth. J. Biol. Chem. 270: 23860-23866, 1995.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer using IFITM1 antibody showing cytoplasmic staining. 

Immunohistochemical analysis of formalin fixed paraffin-embedded human intestinal cancer using IFITM1 antibody showing cytoplasmic staining.