Description

The integrin family includes cell surface receptors for extracellular matrix components as well as receptors involved in various aspects of leukocyte adhesion. Integrin alpha chain 4 (ITGA4) is assigned to 2q31-q32. Interaction between leukemic-cell VLA-4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia.

Catalog Number

IW-PA1046

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to the C-terminal of human ITGA4, identical to the related rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human mammary cancer, human intestinal cancer, HT-1080 cell.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Fernandez-Ruiz, E.; Pardo-Manuel de Villena, F.; Rubio, M. A.; Corbi, A. L.; Rodriguez de Cordoba, S.; Sanchez-Madrid, F. : Mapping of the human VLA-alpha-4 gene to chromosome 2q31-q32. Europ. J. Immun. 22: 587-590, 1992.

2. Jaspers, M.; Zhang, Z.; Marynen, P.; Vekemans, S.; Aly, M. S.; Cuppens, H.; Hillicker, C.; Cassiman, J.-J. : Localization of the genes encoding the alpha-2 and alpha-4 subunits of the human VLA-receptors to chromosome 5q23-31 and 2q31-32 respectively. (Abstract) Cytogenet. Cell Genet. 58: 1870 only, 1991.

3. Zhang, Z.; Vekemans, S.; Aly, M. S.; Jaspers, M.; Marynen, P.; Cassiman, J.-J. : The gene for the alpha-4 subunit of the VLA-4 integrin maps to chromosome 2q31-32. Blood 78: 2396-2399, 1991.

4 Matsunaga, T.; Takemoto, N.; Sato, T.; Takimoto, R.; Tanaka, I.; Fujimi, A.; Akiyama, T.; Kuroda, H.; Kawano, Y.; Kobune, M.; Kato, J.; Hirayama, Y.; Sakamaki, S.; Kohda, K.; Miyake, K.; Niitsu, Y. : Interaction between leukemic-cell VLA-4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia. Nature Med. 9: 1158-1165, 2003. Note: Erratum: Nature Med. 11: 578 only, 2005.

Images

Immunohistochemical analysis of paraffin-embedded sections

Human mammary cancer using ITGA4 antibody

Staining ITGA4 in cytoplasm. DAB chromogenic reaction.

Immunocytochemistry analysis of HT-1080 cell using ITGA4 antibody

Immunohistochemical analysis of paraffin-embedded sections

Human intestinal cancer using ITGA4 antibody