Insulin IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-MA1052 9 ml $258 Yes

Description

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al. (1981) and Harper and Saunders (1981) assigned the insulin gene to 11p15.5 by in situ hybridization.

 

Catalog Number

 

IW-MA1052

Quantity

 

9 ml

Host

 

Mouse

 

Clone

 

ISL-8J

 

Isotype

 

Mouse IgG1

 

Immunogen

 

 

Purity

 

Purified by the goat anti-mouse IgG affinity chromatography.

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Pancreas.

 

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Formalin fixed paraffin embedded tissue sections.

IHC-Fr: Not tested.

ICC: Not tested.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Harper, M. E.; Ullrich, A.; Saunders, G. F. : Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc. Nat. Acad. Sci. 78: 4458-4460, 1981.

2. Owerbach, D.; Bell, G. I.; Rutter, W. J.; Shows, T. B. : The insulin gene is located on chromosome 11 in human. Nature 286: 82-84, 1980.

3. Huerre, C.; Gilgenkrantz, S.; Leonard, C.; Pictet, R.; Kaplan, J. C.; Junien, C. : Regional assignment of the structural gene for insulin to 11p15.1-11p15.5 by deletion mapping. (Abstract) Cytogenet. Cell Genet. 37: 495, 1984.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded rat pancreas using Insulin antibody showing cytoplasmic staining pattern. 

 

 

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