Catalog Number | Size | Price | Availability |
IW-PA1048 | 9 ml | $258 | Yes |
Description
|
Lamins are the major components of the nuclear lamina which
underlies the nuclear envelope of eukaryotic cells. lamin B
is a structural component of the long-sought-after spindle
matrix that promotes microtubule assembly and organization
in mitosis. Inspection of the deduced amino acid sequence of
lamin B revealed the presence in coil 1B of the
alpha-helical domain of a leucine heptad repeat region.
Lamin B assembled into a matrix-like network in mitosis
through a process that depended on the presence of the
guanosine triphosphate-bound form of the small guanosine
triphosphatase Ran.
|
Catalog Number
|
IW-PA1048 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence at the
C-terminal of human Lamin B,different from the related rat
and mouse sequence by three amino acids.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Human mammary cancer
|
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Formalin or acetone fixed frozen sections.
ICC: Acetone fixed cells.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K.
E.; Tan, E. M.; Glass, C. A. :
In vitro
posttranslational modification of lamin B clones from a
human T-cell line. Molec. Cell. Biol. 10:
2164-2175, 1990
2. Tsai, M.-Y.; Wang, S.;
Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R.
D.; Zheng, Y. : A
mitotic lamin B matrix induced by RanGTP required for
spindle assembly. Science 311: 1887-1893,
2006.
|
Images
|
Immunohistochemical analysis of formalin fixed
paraffin-embedded human mammary cancer using Lamin β
antibody. Staining Lamin β in cytoplasm. DAB chromogenic
reaction.
|
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