Description

Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260, 000 base pairs and contains 64 exons.  Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins.  And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

Catalog Number

IW-MA1054

Quantity

9 ml

Host

Mouse

Clone

LAM-26

Isotype

Mouse IgG1

Immunogen

Human laminin

Purity

Purified by the goat anti-mouse IgG affinity chromatography

Conjugate

Unconjugated

Species Reactivity

Human, pig, feline. Not tested in other species.

Positive Control

Skin, kidney, skeletal muscle, small intestine.

Cellular Localization

Basement membrane

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Proteolytic Induced Epitope Retrieval (PIER) (Proteinase K) required on formalin fixed paraffin embedded tissue sections.

IHC-Fr: Acetone fixed frozen sections.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Zhang, X.; Vuolteenaho, R.; Tryggvason, K. : Structure of the human laminin alpha-2-chain gene (LAMA2), which is affected in congenital muscular dystrophy. J. Biol. Chem. 271: 27664-27669, 1996.

2. Ehrig, K.; Leivo, I.; Argraves, W. S.; Ruoslahti, E.; Engvall, E. : Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc. Nat. Acad. Sci. 87: 3264-3268, 1990.

3. Arahata, K.; Hayashi, Y. K.; Mizuno, Y.; Yoshida, M.; Ozawa, E. : Dystrophin-associated glycoprotein and dystrophin co-localisation at sarcolemma in Fukuyama congenital muscular dystrophy. (Letter) Lancet 342: 623-624, 1993.

Images

Immunohistochemical analysis of acetone fixed frozen skin sections using Laminin antibody showing basement membrane staining pattern.