Catalog Number | Size | Price | Availability |
IW-PA1049 | 9 ml | $258 | Yes |
Description
|
MAPK1(ERK2) shares high homology with MAPK3(ERK1).
MAP kinase
phosphatase as a locus of flexibility in a mitogen-activated
protein kinase signaling network. Mitogen-activated
protein (MAP) kinases [also known as Erks] have been
established to function as important mediators of signal
transduction by growth factor receptors. ERK1/ERK2-dependent
activation of endogenous ribosomal transcription, while
inactivation of ERK1/ERK2 causes an equally immediate
reversion to the basal transcription level. ERK1/ERK2 was
found to phosphorylate the architectural transcription
factor UBF at
amino acids 117 and 201 within HMG boxes 1 and 2, preventing
their interaction with DNA. Mutation of these sites
inhibited transcription activation and abrogated the
transcriptional response to ERK1/ERK2.
|
Catalog Number
|
IW-PA1049 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A synthetic peptide mapping at the N-terminal of the human
MAPK1+3, identical to the related rat sequence. |
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Human intestinal cancer
|
Cellular Localization
|
Cytoplasmic/nuclear
|
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Bhalla, U. S.; Ram, P. T.;
Iyengar, R. : MAP
kinase phosphatase as a locus of flexibility in a mitogen-activated
protein kinase signaling network.
Science
297: 1018-1023, 2002.
2. Li, L.; Wysk, M.; Gonzalez, F.
A.; Davis, R. J. :
Genomic loci of human mitogen-activated protein kinases.
Oncogene
9: 647-649, 1994.
|
Images
|
Immunohistochemical analysis of formalin fixed
paraffin-embedded human intestinal cancer using MAPK1-3
antibody. Staining MAPK1-3 in cytoplasm and nucleus. DAB
chromogenic reaction.
|
Related Products