Catalog Number | Size | Price | Availability |
IW-PA1050 | 9 ml | $258 | Yes |
Description
|
Myelin basic protein (MBP) is a major
constituent of the myelin sheath of oligodendrocytes and
Schwann cells in the central nervous system and the
peripheral nervous system, respectively. It is most abundant
in hemopoietic system and contains seven exons distributed
over 32-34 kb. MBP isolated from MS brain may differ in
charge microheterogeneity which would affect antigenic
determinants. MBP is mapped to chromosome 18q22-23. Failure
in this gene expression would be correlated in the central
white matter with extrapyramidal system degeneration signs.
Moreover, it is a candidate autoantigen in the disease
multiple sclerosis. |
Catalog Number
|
IW-PA1050 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide corresponding to a sequence at the
C-terminal of the human MBP, identical to the related rat
and mouse sequence. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Mouse, mouse, rat.
Not tested in other species. |
Positive Control |
Human
liver |
Cellular Localization |
Cytoplasmic |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Suitable. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1.
Marty MC,
Alliot F,
Rutin J,
Fritz R,
Trisler D,
Pessac B.The myelin
basic protein gene is expressed in differentiated blood cell
lineages and in hemopoietic progenitors.
Proc Natl Acad Sci U S A. 2002 Jun 25;
99(13):8856-61.
2.
Streicher R,
Stoffel W.The
organization of the human myelin basic protein gene.
Comparison with the mouse gene.
Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10.
3.
McLaurin JA,
Hafler DA,
Antel JP.Reactivity
of normal T-cell lines to MBP isolated from normal and
multiple sclerosis white matter.
J Neurol Sci. 1995 Feb; 128(2):205-11.
4.
Iester A,
Vignola S,
Callegarini L,
Gimelli G,
Alpigiani MG. [18q
syndrome with deficiency of myelin basic protein (MBP)]
Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5.
5.
Tranquill LR,
Skinner E,
Campagnoni C,
Vergelli M,
Hemmer B,
Muraro P,
Martin R,
McFarland HF,
Campagnoni AT,
Voskuhl RR. Human T
lymphocytes specific for the immunodominant 83-99 epitope of
myelin basic protein: recognition of golli MBP HOG 7.
J Neurosci Res. 1996 Sep 15; 45(6):820-8. |
Images |
Immunohistochemical staining of MBP (IW-PA1050) on formalin fixed paraffin-embedded rat brain sections.
|
Related Products