Catalog Number | Size | Price | Availability |
IW-PA1122 | 9 ml | $258 | Yes |
Description
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Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase,
72-kD, which is also known as gelatinase1 and is
a member of a group of secreted zinc metalloproteases2.
The MMP2 gene is 17 kb long with 13 exons varying in size
from 110 to 901 bp and 12 introns ranging from 175 to 4,350
bp 3, located within a region of human chromosome
16q13. In addition,The extra exons encode the amino acids of
the fibronectin-like domain which has so far been found in
only the 72- and 92-kDa type IV collagenase2.
MMP2,which has a critical role in the binding of
progelatinase A and TIMP4 via the C-terminal hemopexin-like
domain (C domain)5, is functionally associated on
the surface of angiogenic blood vessels6. NOT
only is a likely effector of endometrial menstrual breakdown4,
MMP2 is also effector and regulator of the inflammatory
response7. Moreover, MMP2 could be helpful in
diagnosing Takayasu arteritis.
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Catalog Number
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IW-PA1122 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
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IgG
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Immunogen
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A synthetic peptide corresponding to a sequence at the
C-terminal of human
MMP2,
identical to the related rat sequence.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
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Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
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Human bladder carcinoma,
human
epidermal carcinoma,
Human lymphadenoma, Rat intestine.
|
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
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Store at 2-8
°C.
Do not freeze.
|
Applications
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IHC-P: Formalin fixed paraffin embedded tissue.
IHC-Fr: Formalin or acetone fixed frozen tissue.
ICC: Acetone fixed cultured cells.
|
Limitations
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This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
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Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Nagase, H.; Barrett, A. J.; Woessner, J. F., Jr. :
Nomenclature and
glossary of the matrix metalloproteinases. Matrix
Suppl. 1: 421-424, 1992.
2. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.;
Gerhard, D. S. : On
the structure and chromosome location of the 72- and 92-kDa
human type IV collagenase genes. Genomics 9:
429-434, 1991.
3. Huhtala, P.; Chow, L. T.; Tryggvason, K. :
Structure of the
human type IV collagenase gene. J. Biol. Chem.
265: 11077-11082, 1990.
4. Irwin, J. C.; Kirk, D.; Gwatkin, R. B. L.; Navre, M.;
Cannon, P.; Giudice, L. C. :
Human endometrial
matrix metalloproteinase-2, a putative menstrual proteinase:
hormonal regulation in cultured stromal cells and messenger
RNA expression during the menstrual cycle. J. Clin.
Invest. 97: 438-447, 1996.
5. Bigg, H. F.; Shi, Y. E.; Liu, Y. E.; Steffensen, B.;
Overall, C. M. :
Specific, high affinity binding of tissue inhibitor of
metalloproteinases-4 (TIMP-4) to the COOH-terminal hemopexin-like
domain of human gelatinase A. J. Biol. Chem.
272: 15496-15500, 1997.
6. Brooks, P. C.; Silletti, S.; von Schalscha, T. L.;
Friedlander, M.; Cheresh, D. A. :
Disruption of
angiogenesis by PEX, a noncatalytic metalloproteinase
fragment with integrin binding activity. Cell
92: 391-400, 1998.
7. McQuibban, G. A.; Gong, J.-H.; Tam, E. M.; McCulloch, C.
A. G.; Clark-Lewis, I.; Overall, C. M. :
Inflammation
dampened by gelatinase A cleavage of monocyte
chemoattractant protein-3. Science 289:
1202-1206, 2000.
8. Matsuyama, A.; Sakai, N.; Ishigami, M.; Hiraoka, H.;
Kashine, S.; Hirata, A.; Nakamura, T.; Yamashita, S.;
Matsuzawa, Y. :
Matrix metalloproteinases as novel disease markers in
Takayasu arteritis. Circulation 108:
1469-1473, 2003.
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Images
|
Immunohistochemical analysis of formalin fixed
paraffin-embedded rat intestine using MMP2 antibody
Immunohistochemical analysis of formalin fixed
paraffin-embedded human
bladder carcinoma
using MMP2 antibody
Immunocytochemistry analysis of human
epidermal carcinoma
cell using MMP2 antibody
Immunohistochemical analysis of frozen human lymphadenoma
section using MMP2 antibody.
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