Description

Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase¹ and is a member of a group of secreted zinc metalloproteases². The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp ³, located within a region of human chromosome 16q13. In addition,The extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72- and 92-kDa type IV collagenase². MMP2,which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain)⁵, is functionally associated on the surface of angiogenic blood vessels⁶. NOT only is a likely effector of endometrial menstrual breakdown⁴, MMP2 is also effector and regulator of the inflammatory response⁷. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.

Catalog Number

IW-PA1122

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human MMP2, identical to the related rat sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human bladder carcinoma, human epidermal carcinoma, Human lymphadenoma, Rat intestine.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Formalin fixed paraffin embedded tissue.

IHC-Fr: Formalin or acetone fixed frozen tissue.

ICC: Acetone fixed cultured cells.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Nagase, H.; Barrett, A. J.; Woessner, J. F., Jr. : Nomenclature and glossary of the matrix metalloproteinases. Matrix Suppl. 1: 421-424, 1992.

2. Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. : On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991.

3. Huhtala, P.; Chow, L. T.; Tryggvason, K. : Structure of the human type IV collagenase gene. J. Biol. Chem. 265: 11077-11082, 1990.

4. Irwin, J. C.; Kirk, D.; Gwatkin, R. B. L.; Navre, M.; Cannon, P.; Giudice, L. C. : Human endometrial matrix metalloproteinase-2, a putative menstrual proteinase: hormonal regulation in cultured stromal cells and messenger RNA expression during the menstrual cycle. J. Clin. Invest. 97: 438-447, 1996.

5. Bigg, H. F.; Shi, Y. E.; Liu, Y. E.; Steffensen, B.; Overall, C. M. : Specific, high affinity binding of tissue inhibitor of metalloproteinases-4 (TIMP-4) to the COOH-terminal hemopexin-like domain of human gelatinase A. J. Biol. Chem. 272: 15496-15500, 1997.

6. Brooks, P. C.; Silletti, S.; von Schalscha, T. L.; Friedlander, M.; Cheresh, D. A. : Disruption of angiogenesis by PEX, a noncatalytic metalloproteinase fragment with integrin binding activity. Cell 92: 391-400, 1998.

7. McQuibban, G. A.; Gong, J.-H.; Tam, E. M.; McCulloch, C. A. G.; Clark-Lewis, I.; Overall, C. M. : Inflammation dampened by gelatinase A cleavage of monocyte chemoattractant protein-3. Science 289: 1202-1206, 2000.

8. Matsuyama, A.; Sakai, N.; Ishigami, M.; Hiraoka, H.; Kashine, S.; Hirata, A.; Nakamura, T.; Yamashita, S.; Matsuzawa, Y. : Matrix metalloproteinases as novel disease markers in Takayasu arteritis. Circulation 108: 1469-1473, 2003.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded rat intestine using MMP2 antibody.

Immunohistochemical analysis of formalin fixed paraffin-embedded human bladder carcinoma using MMP2 antibody.

Immunocytochemistry analysis of human epidermal carcinoma cell using MMP2 antibody.

Immunohistochemical analysis of frozen human lymphadenoma section using MMP2 antibody.