Catalog Number | Size | Price | Availability |
IW-PA1207 | 9 ml | $258 | Yes |
Description
|
MMP8 (Matrix metalloproteinase 8) is a member of the family
of matrix metalloproteinases. It is distinct from the
collagenase of skin fibroblasts and synovial cells in
substrate specificity and immunologic crossreactivity. MMP8
was mapped to 11q21-q22. MMP8 is an enzyme that degrades
fibrillar collagens imparting strength to the fetal
membranes, is expressed by leukocytes and chorionic
cytotrophoblast cells. The enzyme exhibits 58% homology to
human fibroblast collagenase and has the same domain
structure. It consists of a 20-residue signal peptide, and
an 80-residue propeptide that is lost on autolytic
activation by cleavage of an M-L bond. MMP8 was found to
possess 57% identity with the deduced protein sequence for
fibroblast collagenase with 72% chemical similarity. Matrix
metalloproteinases (MMPs) have fundamental roles in tumor
progression, but most clinical trials with MMP inhibitors
have not shown improvements in individuals with cancer. MMP8
has a paradoxical protective role in cancer and provides a
genetic model to evaluate the molecular basis of gender
differences in cancer susceptibility.
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Catalog Number
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IW-PA1207 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
Rabbit IgG
|
Immunogen
|
A synthetic peptide corresponding to a sequence at the
N-terminal of human MMP8, different to the related rat
sequence by three amino acids.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
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Human mammary cancer, rat lung. |
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
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IHC-P: Formalin fixed paraffin embedded tissue sections.
IHC-Fr: Not tested.
ICC: Not tested.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1.
Wang, H.; Parry, S.; Macones, G.; Sammel, M. D.; Ferrand, P.
E.; Kuivaniemi, H.; Tromp, G.; Halder, I.; Shriver, M. D.;
Romero, R.; Strauss, J. F., III :
Functionally
significant SNP MMP8 promoter haplotypes and preterm
premature rupture of membranes (PPROM). Hum. Molec.
Genet. 13: 2659-2669, 2004.
2.
Devarajan, P.; Mookhtiar, K.; Van Wart, H.; Berliner, N. :
Structure and
expression of the cDNA encoding human neutrophil collagenase.
Blood 77: 2731-2738, 1991.
3.
Hasty, K. A.; Pourmotabbed, T. F.; Goldberg, G. I.;
Thompson, J. P.; Spinella, D. G.; Stevens, R. M.; Mainardi,
C. L. : Human
neutrophil collagenase: a distinct gene product with
homology to other matrix metalloproteinases. J.
Biol. Chem. 265: 11421-11424, 1990.
4. Balbin, M.; Fueyo, A.; Tester,
A. M.; Pendas, A. M.; Pitiot, A. S.; Astudillo, A.; Overall,
C. M.; Shapiro, S. D.; Lopez-Otin, C. :
Loss of
collagenase-2 confers increased skin tumor susceptibility to
male mice. Nature Genet. 35: 252-257, 2003.
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Images
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Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer using MMP8 antibody showing cytoplasmic staining.
Immunohistochemical analysis of formalin fixed paraffin-embedded rat lung using MMP8 antibody showing cytoplasmic staining.
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