NSE IHC Antibody

 

Ready To Use

 

Catalog Number Size Price Availability
IW-PA1061 9 ml $258 Yes

Description

NSE (neuron specific enolase) also knows as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

 
Catalog Number

 

IW-PA1061
Quantity

 

9 ml

Host

 

Rabbit

 

Clone

 

Polyclonal

 

Isotype

 

IgG

 

Immunogen

 

A peptide mapping at the C-terminus of NSE of human origin, identical to the related mouse and rat sequence.

 

Purity

 

Immunogen affinity purified

 

Conjugate

 

Unconjugated

 

Species Reactivity

 

Human, mouse, rat. Not tested in other species.

Positive Control

 

Brain, pancreas, testis

 

Cellular Localization

 

Cytoplasmic

 

Form

 

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

 

Storage

 

Store at 2-8 °C. Do not freeze.

 

Applications

 

IHC-P: Heat induced epitope retrieval or proteinase K pretreatment is required on formalin fixed paraffin embedded sections. IHC-Tek Epitope Retrieval Solution (Cat# IW-1100) or IHC-Tek Proteinase K Solution (IW-1101) is recommended.

IHC-Fr: Not tested.

ICC: Suitable.

 

Limitations

 

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

 

Precautions

 

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

 

References

 

1. Craig, S. P.; Day, I. N. M.; Thompson, R. J.; Craig, I. W. : Localization of human neurone-specific enolase to chromosome 12p13. (Abstract) Cytogenet. Cell Genet. 51: 980 only, 1989.

2. Craig, S. P.; Day, I. N. M.; Thompson, R. J.; Craig, I. W. : Localisation of neurone-specific enolase (ENO2) to 12p13. Cytogenet. Cell Genet. 54: 71-73, 1990.

3. Mattei, J. F.; Baeteman, M. A.; Mattei, M. G.; Ardissonne, J. P.; Giraud, F. : Regional assignments of CS and ENO2 on chromosome 12. (Abstract) Cytogenet. Cell Genet. 32: 297 only, 1982.

 

Images

 

Immunohistochemical analysis of formalin fixed paraffin-embedded rat brain using NSE antibody showing cytoplasmic staining pattern of neurons.

 

IHC staining of formalin fixed paraffin embedded human pancreas using NSE antibody showing cytoplasmic staining pattern of islet cells.

 

IHC staining of formalin fixed paraffin embedded human testis using NSE antibody showing cytoplasmic staining pattern of leydig cells.

 

 Related Products