Catalog Number | Size | Price | Availability |
IW-MA1083 | 9 ml | $258 | Yes |
Description |
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
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Catalog Number
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IW-MA1083
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Quantity
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9 ml
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Host |
Mouse
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Clone |
IML-83
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Isotype |
Mouse IgG2a
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Immunogen |
Protein A fusion protein.
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Purity |
Purified by the goat anti-mouse IgG affinity chromatography.
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Conjugate |
Unconjugated
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Species Reactivity |
Human, mouse, rat. Not tested in other species.
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Positive Control |
Skin, Hela cells
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Cellular Localization |
Nuclear
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Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted.
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Storage |
Store at 2-8 °C. Do not freeze.
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Applications |
IHC-P: Heat induced epitope retrieval is
required on formalin fixed paraffin embedded sections. IHC-Tek
Epitope Retrieval Solution (IW-1100) is recommended. IHC-Fr: Formalin fixed or acetone fixed
frozen tissues. ICC: Suitable.
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Limitations |
This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.
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Precautions |
Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.
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References |
1. Bravo, R. :
Synthesis of the
nuclear protein cyclin (PCNA) and its relationship with DNA
replication. Exp.
Cell Res. 163: 287-293,
1986. 2. Moldovan,
G.-L.; Pfander, B.; Jentsch, S. :
PCNA controls establishment of sister chromatid cohesion during S phase.
Molec. Cell
23: 723-732, 2006. 3. Webb, G.; Parsons, P.; Chenevix-Trench, G. : Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum. Genet. 86: 84-86, 1990.
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Images |
Immunohistochemical staining of Hela
cells using PCNA
antibody showing nuclear staining pattern. DAB
chromogen substrate.
Immunohistochemical staining of formalin fixed paraffin
embedded human skin using PCNA
antibody showing
nuclear staining pattern. DAB chromogen substrate. |
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