Description

The transcription factor, signal transducer and activator of transcription-3 (STAT-3) is the most pleiotropic member of the signal transducer and activator of transcription (STAT) family of transcription factors and mediates pivotal responses for the cytokine family. The mouse STAT3 gene contains 24 exons and spans 30 kb. The translation initiation codon is in exon 2, and the stop codon is in exon 24. STAT3 is mapped to 17q21, it contributes to various physiological processes. Hepatic STAT-3 signaling is thus essential for normal glucose homeostasis and may provide new therapeutic targets for diabetes mellitus.

Catalog Number

IW-PA1108

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human STAT3, identical to the related rat and mouse sequence.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Human mammary cancer

Cellular Localization

Cytoplasmic, nuclear.

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Suitable.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1.      Bhattacharya, S.; Schindler, C. : Regulation of Stat3 nuclear export. J. Clin. Invest. 111: 553-559, 2003.

2.      Miyoshi, K.; Cui, Y.; Riedlinger, G.; Robinson, P.; Lehoczky, J.; Zon, L.; Oka, T.; Dewar, K.; Hennighausen, L. : Structure of the mouse Stat 3/5 locus: evolution from Drosophila to zebrafish to mouse. Genomics 71: 150-155, 2001.

3.      Inoue, H.; Ogawa, W.; Ozaki, M.; Haga, S.; Matsumoto, M.; Furukawa, K.; Hashimoto, N.; Kido, Y.; Mori, T.; Sakaue, H.; Teshigawara, K.; Jin, S.; Iguchi, H.; Hiramatsu, R.; LeRoith, D.; Takeda, K.; Akira, S.; Kasuga, M. : Role of STAT-3 in regulation of hepatic gluconeogenic genes and carbohydrate metabolism in vivo. Nature Med. 10: 168-174, 2004.

Images

Immunohistochemical analysis of formalin fixed paraffin-embedded human mammary cancer section using STAT3 antibody.