Description

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Catalog Number

IW-MA1102

Quantity

9 ml

Host

Mouse

Clone

VMT-24

Isotype

Mouse IgG1

Immunogen

Pig eye lens vimentin.

Purity

Purified by the goat anti-mouse IgG affinity chromatography.

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Tonsil, skin, smooth muscle.

Cellular Localization

Cytoplasmic

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin embedded sections.

IHC-Fr: Not tested.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986.

2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003

3 Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.

Images

Immunohistochemical analysis of formalin fixed paraffin embedded human spleen using Vimentin antibody.