alpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

IW-MA1107

9 ml

Mouse

Tub-1

Mouse IgG1

Microtubules from chicken embryo brain.

Purified by the goat anti-mouse IgG affinity chromatography.

Unconjugated

Human, rat. Not tested in other species.

Brain

Cytoplasmic

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Store at 2-8 °C. Do not freeze.

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983.

2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985.

Immunohistochemistry staining of formalin fixed paraffin embedded human mammary cancer using alpha-Tubulin antibody showing cytoplasmic staining.