Catalog Number | Size | Price | Availability |
IW-MA1115 | 9 ml | $258 | Yes |
Description
|
The primary site of action of cytochalasin B on cell
motility processes is beta-actin.
Habets et al. (1992) generated hybrids that
harbor only specific regions of human chromosome 7 and
assigned the ACTB locus to 7p15-p12. ACTB and the other
assigned beta-actin-related sequences are dispersed over at
least four different chromosomes including one locus
assigned to the X chromosome.
A mutation of beta-actin
that alters depolymerization dynamics is associated with
autosomal dominant developmental malformations, deafness,
and dystonia.
|
Catalog Number
|
IW-MA1115 |
Quantity
|
9 ml |
Host
|
Mouse
|
Clone
|
AC-15
|
Isotype
|
Mouse IgG1
|
Immunogen
|
slightly modified β-cytoplasmic actin N-terminal peptide,
Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys,
conjugated to KLH. |
Purity
|
Purified by the goat anti-mouse IgG affinity chromatography. |
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Skeletal muscle |
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1. Ng, S.-Y.; Gunning, P.; Eddy,
R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. :
Chromosome 7
assignment of the human beta-actin functional gene (ACTB)
and the chromosomal dispersion of pseudogenes.
(Abstract)
Cytogenet. Cell Genet. 40: 712 only, 1985.
2. Procaccio, V.; Salazar, G.;
Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez,
R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella,
B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. :
A mutation of beta-actin
that alters depolymerization dynamics is associated with
autosomal dominant developmental malformations, deafness,
and dystonia.
Am. J. Hum. Genet.
78: 947-960, 2006.
3. Ng, S.-Y.; Gunning, P.; Eddy,
R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. :
Evolution of the
functional human beta-actin gene and its multi-pseudogene
family: conservation of the noncoding regions and
chromosomal dispersion of pseudogenes.
Molec. Cell. Biol.
5: 2720-2732, 1985.
|
Images
|
Immunohistochemical staining of formalin fixed
paraffin-embedded rat skeletal muscle
using beta-Actin antibody
|
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