Klear Mouse Antibody on Mouse Tissue IHC Detection System

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Immunohistochemistry (IHC) Detection Systems

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Klear Mouse HRP Detection System

Polymer HRP detection system for

Mouse Antibody on Mouse Tissue

With GBI’s Klear Mouse Blocking reagent (Cat. No. D54-15) Mouse smooth muscle actin stained on mouse frozen liver tissue.

No Blocking. Mouse smooth muscle actin stained on mouse frozen liver tissue.

Features

For mouse antibody on mouse tissue
Biotin free system
Clean background and faster turnaround
Choose appropriate polymer-HRP for DAB and AEC

Klear Mouse HRP Detection System is used for visualizing mouse originated primary antibodies on mouse tissue section. Klear Mouse HRP detection system uses Polink-2 Plus technology to achieve ultra sensitive without a noticeable increase in background noise. The system is biotin-free and is provided in easy-to-use convenient dropper bottles.

The system contains block reagents that effectively block the secondary antibody from reacting with endogenous IgG on mouse tissue.

Catalog No.	Product Name	Mouse Blocking Buffer A&B	Chromogen
D52-110	Klear Mouse HRP for DAB bulk kit, 110 ml	Included	Not included
D52-15	Klear Mouse HRP with DAB kit 15ml	Included	DAB
D52-7	Klear Mouse HRP with DAB kit 7ml kit	Included	DAB
D53-110	Klear Mouse HRP for AEC Bulk kit, 110 ml	Included	Not included
D53-15	Klear Mouse HRP with AEC 15ml kit	Included	AEC
D53-7	Klear Mouse HRP with AEC 7ml kit	Included	AEC
D54-110	Klear Mouse Blocking A & B, 110 ml	Included	Not included
D54-15	Klear Mouse Blocking A & B, 15 ml	Included	Not included

Klear Mouse AP Detection System

Polymer AP detection system for

Mouse Antibody on Mouse Tissue

Features

For mouse antibody on mouse tissue with superior sensitivity and clean background
Require only 35 minutes of blocking compared to more than 1 hour blocking from other companies
Biotin free system. No biotin blocking is needed

Klear Mouse AP Detection System is used for visualizing mouse originated primary antibodies on mouse tissue section. Klear Mouse AP detection system uses Polink-2 Plus technology to achieve ultra sensitive without a noticeable increase in background staining. The system is biotin-free and is provided in easy-to-use convenient dropper bottles.

The system contains block reagents that effectively block the secondary antibody from reacting with endogenous IgG on mouse tissue.

Customer may choose Klear Mouse on Mouse AP with Fast Red or AP-Red+ according to the table.

Catalog No.	Product Name	Mouse Blocking Buffer A&B	Chromogen
D50-15	Klear Mouse on Mouse AP Fast Red kit, 15 ml	Included	Fast Red
D50-7	Klear Mouse on Mouse AP Fast Red kit, 7 ml	Included	Fast Red
D51-15	Klear Mouse on Mouse AP AP-Red+ kit, 15 ml	Included	AP-Red+
D51-7	Klear Mouse on Mouse AP AP-Red+ kit, 7 ml	Included	AP-Red+

Polymer-HRP and Polymer AP for Animal Tissue

Rat Antibody on Mouse Tissue

Mouse Antibody on Rat Tissue

Rat-on-Mouse Polymer Detection System

Detecting RAT primary antibody on MOUSE tissue is a very difficult task in research due to cross reactivity. Polink-1 HRP (or AP) RAT-NM (Non-Mouse) kits and Polink-2 HRP (or AP) RAT-NM kits are specially designed to solve this problem. This technology provides excellent specificity to detect rat primary antibody (user supplied) on mouse tissue. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. The secondary antibody was adsorbed to mouse, rabbit and human serum proteins. It also can be used on human and rabbit tissue. Specimen can be frozen or paraffin – embedded tissues, and freshly prepared monolayer cell smears.

One may choose Polink-1 HRP (or AP ) RAT-NM Detection kit for quicker turn around time. Polink-1 system is the ONE step polymer detection system that uses polymeric HRP-linked anti rat secondary antibody to directly detect rat primary antibody bound to the tissue. For ultra sensitivity, one may choose Polink-2 Plus HRP (or AP) Rat-NM kit. Choose appropriate polymer-HRP with DAB or AEC stain according to the table. Chromogen is not provided in Polink-2 AP Plus Rat kit. Refer to page 28-29 to choose preferred chromogen.

Catalog No.	Product Name	Primary Antibody	Chromogen
D35-110	Polink-1 HRP for DAB Rat-NM 110ml bulk	Rat	DAB
D35-15	Polink-1 HRP RAT-NM with DAB, 15 ml	Rat	DAB
D35-6	Polink-1 HRP RAT-NM with DAB, 6 ml	Rat	DAB
D36-110	Polink-1 HRP Rat-NM bulk for AEC, 110 ml	Rat	AEC
D36-15	Polink-1 HRP RAT-NM with AEC, 15 ml	Rat	AEC
D36-6	Polink-1 HRP RAT-NM with AEC, 6 ml	Rat	AEC
D62-110	Polink-1 AP Rat-NM bulk kit, 110 ml	Rat	Not included
D62-15	Polink-1 AP RAT-NM 15ml kit	Rat	Not included
D62-6	Polink-1 AP RAT-NM 6ml kit	Rat	Not included
D46-110	Polink-2 Plus HRP RAT-NM for DAB bulk Kit, 110 ml	Rat	DAB
D46-15	Polink-2 Plus HRP RAT-NM with DAB, 15 ml	Rat	DAB
D46-6	Polink-2 Plus HRP RAT-NM with DAB, 6 ml	Rat	DAB
D48-110	Polink-2 Plus HRP RAT-NM for AEC bulk Kit, 110 ml	Rat	AEC
D48-15	Polink-2 Plus HRP RAT-NM with AEC, 15 ml	Rat	AEC
D48-6	Polink-2 Plus HRP RAT-NM with AEC, 6 ml	Rat	AEC
D67-110	Polink-2 Plus AP RAT-NM Bulk Kit, 110 ml	Rat	Not included
D67-15	Polink-2 Plus AP RAT-NM 15ml kit	Rat	Not included
D67-6	Polink-2 Plus AP RAT-NM 6ml kit	Rat	Not included

Mouse-on-Rat Polymer Detection System

Detecting MOUSE primary antibody on RAT tissue is a very difficult task in research due to cross reactivity. Polink-1 HRP (or AP) MOUSE-NR (Non-Rat) kits and Polink-2 HRP (or AP) MOUSE-NR kits are specially designed to solve the problem. This technology provides excellent specificity to detect mouse primary antibody (user supplied) on rat tissue. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. The secondary antibody was adsorbed to rat, rabbit and human serum proteins. It also can be used on human and rabbit tissue. Specimen can be frozen or paraffin – embedded tissues, and freshly prepared monolayer cell smears.

One may choose Polink-1 HRP (or AP ) MOUSE-NR Detection kit for quicker turn around time. Polink-1 system is the ONE step polymer detection system that uses polymeric HRP-linked anti mouse secondary antibody to directly detect mouse primary antibody bound to the tissue. For ultra sensitivity, one may choose Polink-2 Plus HRP (or AP) MOUSE-NR kit. Choose appropriate polymer-HRP with DAB or AEC stain according to the table. Chromogen is not provided in Polink-2 Plus AP MOUSE-NR kit. Refer to page 28 to choose preferred chromogen.

Catalog No.	Product Name	Primary Antibody	Chromogen
D55-110	Polink-1 HRP for DAB Mouse-NR 110ml bulk	Mouse	DAB
D55-15	Polink-1 HRP MOUSE-NR with DAB, 15 ml	Mouse	DAB
D55-6	Polink-1 HRP MOUSE-NR with DAB, 6 ml	Mouse	DAB
D56-110	Polink-1 HRP Mouse- NR for AEC bulk, 110 ml	Mouse	AEC
D56-15	Polink-1 HRP MOUSE-NR with AEC, 15 ml	Mouse	AEC
D56-6	Polink-1 HRP MOUSE-NR with AEC, 6 ml	Mouse	AEC
D57-110	Polink-1 AP Mouse- NR bulk kit, 110 ml	Mouse	Not included
D57-15	Polink-1 AP MOUSE-NR 15ml kit	Mouse	Not included
D57-6	Polink-1 AP MOUSE-NR 6ml kit	Mouse	Not included
D58-110	Polink-2 Plus HRP MOUSE-NR for DAB Bulk Kit, 110 ml	Mouse	DAB
D58-15	Polink-2 Plus HRP MOUSE-NR with DAB, 15 ml	Mouse	DAB
D58-6	Polink-2 Plus HRP MOUSE-NR with DAB, 6 ml	Mouse	DAB
D59-110	Polink-2 Plus HRP MOUSE-NR for AEC Bulk Kit, 110 ml	Mouse	AEC
D59-15	Polink-2 Plus HRP MOUSE-NR with AEC, 15 ml	Mouse	AEC
D59-6	Polink-2 Plus HRP MOUSE-NR with AEC, 6 ml	Mouse	AEC
D65-110	Polink-2 Plus AP MOUSE-NR Bulk Kit, 110 ml	Mouse	Not included
D65-15	Polink-2 Plus AP MOUSE-NR 15ml kit	Mouse	Not included
D65-6	Polink-2 Plus AP MOUSE-NR 6ml kit	Mouse	Not included

How to Order

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Credit Card Order need to call us at 443-686-9618
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