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Immunohistochemistry (IHC) Detection Systems
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Polink-2 HRP Detection System
Two-step polymer-HRP detection system
for Mouse & Rabbit antibodies
Comparison of immunostaining intensity using Polink-2 HRP detection kit with other source polymer detection kit. A and B are colon cancer stained by Ms anti-CEA. C and D are breast cancer stained by Ms anti-ER. B and D are detected using Polink-2 HRP (cat. No. D22-15) kit. A and C are detected using other supplier’s polymer kit. The intensity of immunostaining using Polink-2 HRP kit is significantly stronger than other source polymer kit.
Features
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2nd generation of polymer HRP (non-biotin) system, avoids endogenous biotin background completely
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More sensitive than Polink-1 HRP system with clean background
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Two-step polymer detection system
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Compatible with either manual or automated systems
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Better results on difficult-to-work antibodies and nuclear staining
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Save cost due to further dilution of primary antibody
Polink-2 HRP Detection Kit is the second generation of polymer-based detection system that uses proprietary polymer helper and polymeric HRP-linked secondary antibody conjugates to achieve super sensitivity and consistent immunostaining.
This technology provides excellent sensitivity and high specificity. It is about 3-4 times more sensitive than the 1-step polymer detection system and biotin-streptavidin system. It helps to achieve better results on difficult-to-work antibody and nuclear staining. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Since it has higher sensitivity than other conventional methods and the 1-step polymer detection system (Polink-1 HRP system), users may need to further dilute the concentration of primary antibodies if switching from conventional three-step or Polink-1 HRP detection methods.
Refer to the following table to choose Polink-2 HRP for DAB or Polink-2 HRP for AEC. For single species capacity or ultra sensitivity, please choose the third generation of polymer detection system, Polink-2 Plus HRP detection system.
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Catalog No. |
Product Name |
Primary Antibody |
Chromogen |
|
D22-110 |
Polink-2 HRP Broad for DAB Bulk kit, 110 ml |
Mouse & Rabbit |
Not included |
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D22-60 |
Polink-2 HRP Broad for DAB Bulk kit, 60 ml |
Mouse & Rabbit |
Not included |
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D22-15 |
Polink-2 HRP Board with DAB, 15 ml |
Mouse & Rabbit |
DAB |
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D22-6 |
Polink-2 HRP Board with DAB, 6 ml |
Mouse & Rabbit |
DAB |
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D23-110 |
Polink-2 HRP Board for AEC Bulk kit, 110 ml |
Mouse & Rabbit |
Not included |
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D23-60 |
Polink-2 HRP Board for AEC Bulk kit, 60 ml |
Mouse & Rabbit |
Not included |
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D23-15 |
Polink-2 HRP Board with AEC, 15 ml |
Mouse & Rabbit |
AEC |
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D23-6 |
Polink-2 HRP Board with AEC, 6 ml |
Mouse & Rabbit |
AEC |
Polink-2 AP Detection System
Two-step polymer-AP detection system
for Mouse & Rabbit antibodies
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Ms. Anti-ER stained breast cancer using Polink-2 AP kit (Cat No D24-110) with Fast red. |
Rb. Anti-PLAP stained placenta using Polink-2 AP kit (Cat No. D24-110) with Fast Red. |
Features
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2nd generation of polymer AP (non-biotin) system, avoids endogenous biotin background completely
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More sensitive than Polink-1 AP system with clean background
-
Two-step polymer detection system
-
Compatible with either manual or automated systems
-
Better results on difficult-to-work antibodies and nuclear staining
-
Save cost due to further dilution of primary antibody
Polink-2 AP detection kit is the second generation polymer-based detection system that uses proprietary polymer helper and polymeric alkaline phosphatase (AP)-linked secondary antibody conjugates to achieve super sensitivity and consistent immunostaining.
This technology provides excellent sensitivity and high specificity. It is about 3-4 times more sensitive than the 1-step polymer-AP detection system and biotin-streptavidin system. It helps to achieve better results on difficult-to-work antibody and nuclear staining. It is a biotin-free system, so therefore overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotin. Since it has higher sensitivity than other conventional methods and 1-step polymer detection system (Polink-1 AP system), users may need to further dilute the concentration of primary antibodies if switching from the conventional three-step or Polink-1 AP detection methods.
Only broad spectrum detection kits are available in Polink-2 AP detection system. For working with single species or ultra sensitivity, please choose the third generation of polymer detection system, Polink-2 Plus AP detection system.
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Catalog No. |
Product Name |
Primary Antibody |
Chromogen |
|
D24-110 |
Polink-2 AP Board Bulk Kit, 110 ml |
Mouse & Rabbit |
Not included |
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D24-15 |
Polink-2 AP 15ml Kit |
Mouse & Rabbit |
Not included |
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D24-6 |
Polink-2 AP 6ml kit |
Mouse & Rabbit |
Not included |
How to Order
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PO can be Emailed to sales@ihcworld.com or Faxed to 443-638-0394
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Credit Card Order need to call us at 443-686-9618
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Online Order can be placed on our Online Store at http://store.ihcworld.com/
Related Products
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- Polink-1 Polymer System
- Polink-2 Polymer System
- Polink-2 Plus Polymer System
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- Mounting Medium
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