ABC of Safety in the Biological Sciences
BRILLIANT CRESYL BLUE (Colour Index No. 51010) (C15H16N3OCl)
SYNONYMS
None known.
CHARACTERISTICS
Dark green powder. Quinoneimine group of dyes.
INCOMPATIBILITY
Heating produces toxic fumes of chlorides and oxides of
nitrogen.
HEALTH HAZARD DATA
There is insufficient data available on the toxic effects of
this dye. However, other dyes in this group are known to be
potential carcinogens, highly toxic and irritants.
HANDLING and GENERAL PRECAUTIONS
Avoid skin and eye contact.
Avoid inhalation or ingestion of the powder.
Keep away from heat.
Wash hands thoroughly after handling.
Use a fume hood to minimise exposure to this substance. Wear
protective clothing. A long sleeved laboratory coat or gown,
rubber gloves, safety goggles and a face mask as a minimum
standard.
TREATMENTS
Skin immediately remove contaminated clothing and wash the
affected area with soap or mild detergent and large amounts of
water until all evidence of the chemical has been removed
(approximately 15 minutes). If irritation persists seek medical
attention. Wash contaminated clothing before reuse.
Eyes immediately wash the affected eye with large amounts of
water until all evidence of the chemical has been removed
(approximately 15 minutes). If irritation or pain persist seek
medical attention.
Inhalation remove from the area of exposure to fresh air. If
breathing has ceased apply artificial respiration. Keep warm and
allow to rest. If irritation persists or develops seek medical
attention.
Ingestion wash out the mouth thoroughly with water and give
water to drink. Seek immediate medical attention.
STORAGE
Store in a cool dry place.
DISPOSAL
SPILLS:
On skin and clothing - wash skin with strong soap solution
immediately. Rinse thoroughly. Contaminated clothing should be
removed, dried and washed with strong soap solution or
destroyed. It may be necessary to destroy shoes by burning.
Small spills - absorb liquid on paper towels. Brush solids on to
paper. Place in an iron pan and allow evaporation in a fume
hood. Add crumpled paper and burn. Wash spill site with a strong
soap solution.
Large spills - cover with sand and soda ash mixture (90/10). Mix
and shovel into a cardboard box. Pack with a large excess of
crumpled paper. Burn in an open pit or incinerator equipped with
an afterburner and scrubber.
PACKAGE LOTS: Either
1. Pour or sift on to a thick layer of sand and soda ash mixture
(90/10). Mix, and shovel into a heavy paper box with much paper
packaging. Burn in an incinerator. The fire may be augmented
with scrap wood. Stay on the upwind side.
OR
2. Dissolve in a flammable solvent and spray into the firebox of
an incinerator equipped with an afterburner and scrubber.
ENVIRONMENT
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