ABC of Safety in the Biological Sciences



LIGHT GREEN SF Colour Index No. 42095 (C37H34N2O9S3Na2)

Acid green extra, acid green 5.
Use a fume hood.
Potential carcinogen.
Avoid skin and eye contact.
Avoid inhalation or ingestion of the powder.
Keep away from heat or naked flames.
Wash hands thoroughly after handling.
Green powder. Diaminotriarylmethane group of dyes.
This dye is a potential carcinogen and should be handled with great care. It is a toxic substance and an irritant. It is a combustible powder and should not be handled close to heat or a naked flame.
Skin ­ remove contaminated clothing and immediately wash the affected area with soap or mild detergent and large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists or dermatitis develops seek medical attention. Wash contaminated clothing before re­use.
Eyes ­ immediately wash the affected eye with large amounts of water until all evidence of the chemical has been removed (approximately 15 minutes). If irritation or pain persist seek immediate medical attention.
Inhalation ­ remove from the area of exposure to fresh air. If breathing has ceased apply artificial respiration. Give oxygen if necessary. Keep warm and allow to rest. If irritation or discomfort persist seek immediate medical attention.
Ingestion ­ wash out the mouth thoroughly with water. Seek immediate medical advice.
Heating produces toxic fumes of sulphur and nitrogen compounds. Can react vigorously with oxidising materials.
Store in a cool, dry atmosphere away from heat and oxidising materials.
HANDLING Use a fume hood to keep the level of exposure to a minimum. Wear protective clothing. A long sleeved laboratory coat or gown, rubber gloves, safety goggles and a face mask as a minimum standard.
Leather gloves, heavy face shield and laboratory coat. Work from behind a barricade (body shield or wall). Avoid unnecessary heat, friction or impact.
Absorb the liquid with paper or vermiculite. If it is a solid, dampen then brush on to paper with great care. Place in a plastic bag and remove outside for burning.
Sponge up with water, followed by decontamination with 10% ammonium ceric nitrate solution. 'Kill', by adding to a greater stoichiometric amount of ammonium ceric nitrate solution with agitation sufficient to provide suspension of all solids. Cool the reaction. The organic azides and heavy metal azides are explosive. Alkali and alkaline earth azides are not considered explosives under normal laboratory conditions. Keep the stock of azides very low. Stamp the date of receipt upon the package.


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