ABC of Safety in the Biological Sciences



PHLOXINE B (Colour Index No. 45410) (C20H2O5Br4Cl4Na2)

Phloxine TA, N, BF, RB, TB, BB, cyanosine, eosin 10B, magdala red, acid red 92, eosine blue.
Avoid eye contact.
Avoid inhalation or ingestion of the powder.
Keep away from heat.
Wash hands thoroughly after handling.
Reddish­brown powder. Xanthene group of dyes.
Phloxine is highly toxic via the intravenous route. Other data has not been quantified although because of its chemical structure it is assumed to be an eye and skin irritant. It causes foetal death in animals.
Skin ­ remove contaminated clothing then wash the affected area with soap or mild detergent and large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek medical advice. Wash contaminated clothing before re­use.
Eyes ­ immediately wash the affected eye with large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek immediate medical attention.
Inhalation ­ remove from the area of exposure to fresh air. If breathing has ceased apply artificial respiration. Give oxygen if required. Keep warm and allow to rest. Seek immediate medical attention.
Ingestion ­ wash out the mouth thoroughly with water. If the victim is conscious, induce vomiting by touching a finger to the back of the throat. Seek immediate medical attention.
Heating produces toxic fumes of chlorine, bromine and sodium oxide.
Store in a cool, dry atmosphere away from heat.
Use a fume hood to keep the level of exposure to a minimum. Wear protective clothing. A long sleeved laboratory coat or gown, rubber gloves, safety goggles and a face mask as a minimum standard.
Rubber gloves, self contained breathing apparatus and laboratory coat.
Eliminate all sources of ignition. Absorb on paper towels or with vermiculite. Place on an iron, glass or plastic dish in a fume hood. Allow to evaporate. burn the paper or vermiculite. Wash the site with a soapy solution.
1 Pour on to vermiculite, sodium bicarbonate or sand-soda ash mixture (90/10). If a fluoride is present, add slaked lime to the mixture. Mix and shovel into paper boxes. place in an open incinerator, cover with scrap wood and paper then ignite with a torch or fuse. Stay on the upwind side.
2 Dissolve in a flammable solvent. Spray into a fire box of an incinerator equipped with an afterburner and scrubber.


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