ABC of Safety in the Biological Sciences



PYRONIN G (Colour Index No. 45005) (C17H19N2OCl)

Pyronin Y.
Use a fume hood. Potential carcinogen.
Avoid skin and eye contact.
Avoid inhalation or ingestion of the powder.
Keep away from heat.
Keep away from strong oxidising agents.
Keep the container tightly sealed.
Wash hands thoroughly after handling.
Lustrous green to brown crystals with a slight odour. Xanthene group of dyes.
Pyronin is a suspected carcinogen and must be handled with care. Use a fume hood. It is a toxic substance when ingested and a skin, eye and respiratory tract irritant.
Skin ­ immediately remove contaminated clothing and wash the affected area with soap or mild detergent and large amounts of water until all evidence of the chemical has been removed (approximately 15 minutes). If irritation persists seek medical attention. Wash contaminated clothing before re­use.
Eyes ­ immediately wash the affected eye with large amounts of water until all evidence of the chemical has been removed (approximately 15 minutes). If irritation or pain persist seek medical attention.
Inhalation ­ remove from the area of exposure to fresh air. If breathing has ceased apply artificial respiration. Keep warm and allow to rest. If irritation persists or develops seek medical attention.
Ingestion ­ wash out the mouth thoroughly with water and give water to drink. Seek immediate medical advice.
Heating produces toxic fumes of carbon, nitrogen and chlorides.
Store in a cool, dry atmosphere away from heat.
Use a fume hood to minimise exposure to this substance. Wear protective clothing. A long sleeved laboratory coat or gown, rubber gloves, safety goggles and a face mask as a minimum standard.
Rubber gloves, self contained breathing apparatus and laboratory coat.
Eliminate all sources of ignition. Absorb on paper towels or with vermiculite. Place on an iron, glass or plastic dish in a fume hood. Allow to evaporate. burn the paper or vermiculite. Wash the site with a soapy solution.
1 Pour on to vermiculite, sodium bicarbonate or sand-soda ash mixture (90/10). If a fluoride is present, add slaked lime to the mixture. Mix and shovel into paper boxes. place in an open incinerator, cover with scrap wood and paper then ignite with a torch or fuse. Stay on the upwind side.
2 Dissolve in a flammable solvent. Spray into a fire box of an incinerator equipped with an afterburner and scrubber.


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