ABC of Safety in the Biological Sciences



SUDAN IV (Colour Index No. 26105) (C24H20N4O)

Scarlet red, oil red IV, fat ponceau, fat ponceau R, OR or LB, cerotin ponceau 3B, Sudan red IV, solvent red 24.
Mutagen ­ not to be handled by pregnant staff.
Use a fume hood ­ potential carcinogen.
Avoid skin and eye contact.
Keep away from heat.
Wash hands thoroughly after handling.
Dark red brown powder. Disazo group of dyes.
Sudan IV is an animal mutagen and a potential carcinogen. It must never be handled by staff who are pregnant and should only be handled beneath a fumehood. It is highly toxic by the intravenous route but has not been quantified through other routes. It is an eye and respiratory tract irritant.
Skin ­ remove contaminated clothing then wash the affected area with soap or mild detergent and large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek medical advice. Wash contaminated clothing before re­use.
Eyes ­ immediately wash the affected eye with large amounts of water until all evidence of the dye has been removed (approximately 15 minutes). If irritation persists seek immediate medical attention.
Inhalation ­ remove from the area of exposure to fresh air. If breathing has ceased apply artificial respiration. Give oxygen if required. Keep warm and allow to rest. Seek immediate medical attention.
Ingestion ­ wash out the mouth thoroughly with water and give water to drink. If the victim is conscious induce vomiting by touching a finger to the back of the throat. Seek immediate medical attention.
Heating produces toxic fumes of nitrogen compounds.
Store in a cool, dry atmosphere away from heat.
Use a fume hood to keep the level of exposure to a minimum. Wear protective clothing. A long sleeved laboratory coat or gown, rubber gloves, safety goggles and a face mask as a minimum standard.
Leather gloves, heavy face shield and laboratory coat. Work from behind a barricade (body shield or wall). Avoid unnecessary heat, friction or impact.
Absorb the liquid with paper or vermiculite. If it is a solid, dampen then brush on to paper with great care. Place in a plastic bag and remove outside for burning.
Sponge up with water, followed by decontamination with 10% ammonium ceric nitrate solution. 'Kill', by adding to a greater stoichiometric amount of ammonium ceric nitrate solution with agitation sufficient to provide suspension of all solids. Cool the reaction. The organic azides and heavy metal azides are explosive. Alkali and alkaline earth azides are not considered explosives under normal laboratory conditions. Keep the stock of azides very low. Stamp the date of receipt upon the package.


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