ABC of Safety in the Biological Sciences
TRYPAN BLUE (Colour Index No. 23850) (C34H24N6O14S4Na4)
SYNONYMS
Chlorazol blue 3B, benzo blue 3B, dianil blue H3G, Congo blue
3B, naphthamine blue 3BX, benzamine blue 3B, azidine blue 3B,
Niagara blue 3B, diamine blue 3B.
GENERAL PRECAUTIONS
Teratogen must not be handled by staff who are pregnant.
Carcinogen use a fume hood.
Avoid skin and eye contact.
Avoid inhalation or ingestion of the powder.
Keep away from heat.
Wash hands thoroughly after handling.
CHARACTERISTICS
Bluegrey powder, disazo group of dyes.
HEALTH HAZARD DATA
This dye is a known animal carcinogen and a teratogen. It must
never be handled by staff who are pregnant and must only be
handled in a fume hood when the ventilation system is working
efficiently. It is a toxic substance and an eye irritant.
TREATMENTS
Skin remove contaminated clothing then wash the affected area
with soap or mild detergent and large amounts of water until all
evidence of the dye has been removed (approximately 15 minutes).
If irritation persists seek medical advice. Wash contaminated
clothing before reuse.
Eyes immediately wash the affected eye with large amounts of
water until all evidence of the dye has been removed
(approximately 15 minutes). If irritation persists seek
immediate medical attention.
Inhalation remove from the area of exposure to fresh air. If
breathing has ceased apply artificial respiration. Give oxygen
if required. Keep warm and allow to rest. Seek immediate medical
attention.
Ingestion wash out the mouth thoroughly with water and seek
immediate medical attention.
INCOMPATIBILITY
Heating produces toxic fumes of sulphur and nitrogen compounds.
STORAGE
Store in a cool, dry atmosphere away from heat.
HANDLING
Use a fume hood to keep the level of exposure to a minimum. Wear
protective clothing. A long sleeved laboratory coat or gown,
rubber gloves, safety goggles and a face mask as a minimum
standard.
DISPOSAL
WEAR:
Leather gloves, heavy face shield and laboratory coat. Work from
behind a barricade (body shield or wall). Avoid unnecessary
heat, friction or impact.
SPILLS:
Absorb the liquid with paper or vermiculite. If it is a solid,
dampen then brush on to paper with great care. Place in a
plastic bag and remove outside for burning.
OR
Sponge up with water, followed by decontamination with 10%
ammonium ceric nitrate solution. 'Kill', by adding to a greater
stoichiometric amount of ammonium ceric nitrate solution with
agitation sufficient to provide suspension of all solids. Cool
the reaction. The organic azides and heavy metal azides are
explosive. Alkali and alkaline earth azides are not considered
explosives under normal laboratory conditions. Keep the stock of
azides very low. Stamp the date of receipt upon the package.
ENVIRONMENT:
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