ABC of Safety in the Biological Sciences




All cylinders of gas should be correctly identified in accordance with International Regulations and used only for the purpose for which they are designed. Any poorly identified cylinder should be returned to the supplier for credit.

General Precautions
Before using a gas check the label to see that it is the correct gas for your requirements. Never use a gas for any purpose other than that for which it is purchased. Never maltreat valves, gauges or cylinders. If leaks in the system are suspected check by applying detergent and water only to the area suspected of leaking. Stiff valves should never be forced. Any difficult cylinder should be returned to the supplier with an explanatory note. All cylinders and gauges for flammable gases have left­hand threads while non­flammable have right hand. Never completely empty a gas cylinder. Do not open valves too rapidly ­ it could cause overheating of the regulator leading to an explosion. Cylinders must be stored and constrained in a suitable rack or stand to prevent falling. Always store in a cool well ventilated location. Couple cylinders to equipment only through a regulator. Always plan for any emergency ­ keep appropriate emergency equipment on hand.

Commonwealth Industrial Gases (1986)
The Safe Handling of Gases.
Adelaide, South Australia.


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