Histopathology Artifacts Quiz



This slide shows displacement of a trabecula of cancellous bone which is lying over the marrow. This artefact can also affect collagen, elastic, or reticular fibres causing them to become realigned in the direction of cutting. Displacement can be caused by a dull knife, excessively rough sectioning, by using an embedding media which is too soft for the density of the tissue being cut, or by careless flotation technique. Other causes include: incorrect blotting technique and poor adhesion of sections leading to lifting during staining or coverslipping. This is what happened in this instance. The correct answer is displacement of tissue components.

© Roy C. Ellis 2004