Histopathology Artifacts Quiz



This micrograph shows high background staining. In immunohistochemistry this can be attributed to several factors. The most common cause relates to the use of incorrectly titrated or diluted antibodies. Other causes of background staining relate to the presence, in tissues, of both normal and abnormal tissue components. Such background is often regarded as non-specific staining whereas it results from the presence of high normal levels of the antigen under investigation. This micrograph shows strong staining of IgA in mesangial cells while normal serum IgA is demonstrated in the capillaries. Answer high background staining.

© Roy C. Ellis 2004