Prepared by
ROY ELLIS
IMVS Division of Pathology
The Queen Elizabeth Hospital
Woodville Road, Woodville, South Australia 5011
Email: roy.ellis@imvs.sa.gov.au
Acid fast bacilli stained
by the Ellis and Zabrowarny technique
Acid fast bacilli
stained with the fluorescent Kuper and May method
Acid fast bacilli
stained by the Ziehl-Neelsen technique
Aldehyde fuchsin stain
for pancreatic B-cells
Aluminium in
undecalcified bone stained by Irwin's method
Alizarin red S
for calcium in a section of kidney
Amalgam tattoo in
a section of gingiva stained haematoxylin and eosin
Amyloid stained
with Congo red
Amyloid stained
with thioflavine T
Anthrax stained
with Jensen's Picro-Carmine-Gram Method
Aspergillus
fumigatus stained with Grocott's Methenamine Silver
Astrocyte
demonstrated by the immunoperoxidase method for GFAP (Glial
fibrillary acidic protein)
ßA4 protein in a
section of brain from a case of Alzheimer's disease
Best's Carmine
method to stain Glycogen
Bone, undecalcified
under polarized light to demonstrate differences between lamellar
bone and collagen
Bone marrow, human
stained using Jenner-Giemsa
Cajal's gold sublimate
method used to demonstrate neurofibrillary tangles
Calcium in a
section of kidney stained with alizarin red S
Calcium in
undecalcified bone by von Kossa method
Carbon and silica
in a section of lung stained haematoxylin and eosin
Chloroacetate-esterase activity used for demonstrating mast
cells
Cholesterol and
esters stained using the Bromine-sudan black technique
Cholesterol and esters
stained using the perchloric acid-naphthaquinone (PAN) method
Chromotrope-aniline
blue technique for Mallory bodies in human liver
Collagen in
trabecullar bone under polarized light
Collagen stained
by Masson trichrome
Collagen stained by
M.S.B.
Collagen TEM
x20,000
Congo red method
staining amyloid
Copper
demonstrated using the rhodanine technique
Cryptococcus
neoformans stained with crystal violet
DNA in human
liver stained using the Feulgen technique
DNA in an
anaplastic tumour stained by the Feulgen technique
DNA and RNA in
dorsal root ganglion stained using the gallocyanin-chromalum
technique
DNA and RNA in human
liver stained using methyl green-pyronin
Elastic laminae
stained with Verhoeff's iron haemtoxylin
Elastin TEM
x35,000
Ellis and Zabrowarny
technique for acid fast bacilli
Ferric iron
demonstrted using Perl's prussian blue
Feulgen stain
for DNA in human liver
Feulgen stain
for DNA in an anaplastic tumour
Fibrin stained by
M.S.B.
Fibroblast by TEM
x8,000
Formalin pigment
in a section of spleen
Gallocyanin-chromalum
for DNA and RNA in dorsal root ganglion
Giardia Lamblia
in a duodenal aspirate stained by Papanicolaou's method
Giemsa stain to
demonstrate Toxoplasma gondii
GFAP (Glial fibrillary
acidic protein) in an astrocyte demonstrated by the
immunoperoxidase method
Glycogen
stained with Best's Carmine
Gram stain for
Nocardia asteroides
Grimelius technique
staining pancreatic A-cells
Grocott's Methenamine
Silver
Gouty tophus
Haematoxylin and
eosin - Formalin pigment in a section of spleen
Haematoxylin and
eosin - Section of gingiva showing 'amalgam tattoo'
Haematoxylin and eosin
- Gouty tophus
Haematoxylin and eosin
- Seromucous glands of bronchus
Hale's colloidal iron
staining hyaluronic acid in human angiosarcoma
Heidenhain's iron
haematoxylin demonstrating muscle striations
Hepatitis B (Australia
antigen) stained with the Orcein technique
HMB-45 section of
malignant melanoma stained by streptavidin-biotin alkline
phosphatase fast red TR
Hyaluronic acid
stained using Hale's colloidal iron technique
Irwin's method
for aluminium in undecalcified bone
Jenner-Giemsa
stain for bone marrow
Jensen's
Picro-Carmine-Gram to stain Anthrax
Keratin stained
with Masson trichrome
Kuper and May
fluorescent method for acid fast bacilli
Lamellar bone in
undecalcified section under polarized light
Lindner's technique
for nucleolar organizing regions
Luxol fast blue method
for myelinated axons
Macchiavello technique
for viral inclusion bodies
Malignant melanoma
treated with HMB-45 and stained by streptavidin-biotin alkline
phosphatase fast red TR
Mallory bodies
stained using the chromotrope-aniline blue technique
Masson-Fontana
demonstrating melanin pigment
Masson trichrome
staining collagen, muscle and keratin
Mast cell granules
stained with acidified toluidine blue
Mast cells using
chloroacetate esterase activity
Measles virus
inclusion bodies stained with the Macchiavello technique
Melanin pigment
stained with the Masson-Fontana technique
Metachromasia with
toluidine blue
Metachromasia
oxidation in potassium permanganate followed by toluidine blue
Methyl green-pyronin
for DNA and RNA in human liver
M.S.B. staining
fibrin and collagen
Mucin stained
with Southgate's mucicarmine
Mucosubstances
Muscle striations
demonstrated with Heidenhain's iron haematoxylin
Muscle stained
with Masson trichrome
Myelinated axons
stained with the luxol fast blue method
Neurofibrillary
tangles demonstrated by Cajal's gold sublimate method
Neutral fat deposits
stained with the Nile blue sulfate method
Neutral fats
stained with oil red O in isopraponal
Nile blue sulfate
method to differentiate between neutral fats, oils and
cholesterol esters and fatty acids, lipofuschins and phospholipids
Nocardia
asteroides stained with Gram stain
Nucleolar organizing
regions using the technique of Lindner
Oil red O for
Neutral fats
Orcein tecnique for
Australia Antigen (hepatitis B)
Pancreatic A-cells
stained using the Grimelius technique
Pancreatic B-cells
stained with a modified aldehyde fuchsin
Paneth cell granules
stained with phloxine-tartrazine in smll intestine
Perchloric
acid-naphthaquinone (PAN) method for cholesterol and cholesteryl
esters
Perl's prussian blue
for ferric iron
Phloxine-tartrazine
technique for Paneth cell granules in small intestine
Phospholipids
using sudan black with bromination
Pituitary stained
with the Orange G/Acid fuchsin/Light green technique
Pneumocystis carinii
stained with Grocott's Methenamine Silver
Rhodanine technique
for the demonstration of copper
RNA and DNA in
dorsal root ganglion stained using the gallocyanin-chromalum
technique
RNA and DNA in human
liver stained using methyl green-pyronin
Schistosoma
haematobium
in a cervical smear stained with Papanicolaou's
method
Seromucous glands of
bronchus stained haematoxylin and eosin
Silica and carbon
in a section of lung stained haematoxylin and eosin
Small intestine
stained for Paneth cell granules using the phloxine-tartrazine
method
Southgate's
mucicarmine stain for mucin
Spirochates
stained by the Warthin-Starry technique
Streptavidin-biotin
alkline phosphatase fast red TR used to stain malignant melanoma
after treatment with HMB-45
Strongyloides
stercoralis in a sputum stained with Papanicolaou's method
Sudan black with
bromination for cholesterol and cholesterol esters
Thioflavine T,
fluorescent method for amyloid
Toluidine blue,
acidified for staining mast cell granules
Toluidine blue,
metachromatic reaction with cartilage in Guinea pig trachea
Toluidine blue,
metachromatic reaction with cartilage in Guinea pig trachea after
oxidation in potassium permanganate
Toxoplasma gondii
stained with Giemsa stain
Verhoeff's iron
haemtoxylin for elastic laminae
Viral inclusion bodies
stained with the Macchiavello technique
von Kossa for the
demonstration of mineralized calcium in undecalcified bone
Warthin-Starry
technique for Spirochaetes
Ziehl-Neelsen technique
for acid fast bacilli
© Roy C. Ellis 2004