(A) Human adrenal cortex (post mortem): a frozen section of tissue fixed in buffered formalin, stained with Sudan black in ethanol-water after bromination and counterstained with nuclear fast red. (B) A control section of adrenal prepared and stained as for (A) but without bromination.

A                                                                 B

Result: Cholesterol, cholesteryl esters and phospholipids - blue/black in section A but unstained in section B