Problems in Histopathological Technique


Prepared by


IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011





Did you know that when solutions are heated in a microwave for fixation or staining there can be marked differences in temperature between the top and bottom layers of the solution?

That difference can be as much as 15°C.
So take care when using microwave techniques that the recommended temperature is the one that you are using. A microwave that has a means for inserting a temperature probe which can be placed in the solution at the level of the section is the best insurance. Otherwise a frequent check of the temperature after stirring the solution should do the trick.

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© Roy C. Ellis 2002