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Problems in Histopathological Technique
Prepared by
ROY ELLIS
IMVS Division of Pathology
The Queen Elizabeth Hospital
Woodville Road, Woodville, South Australia 5011
Email: roy.ellis@imvs.sa.gov.au
Poor staining or lack of staining with alcian blue dissolved in acetic acid and commonly used for staining mucin.
Alcian blue is said to denature in acid solution over a period of time, the stronger the acid component the sooner denaturation occurs. For example it is said that in a 3% acetic acid solution alcian blue denatures in approximately 1 month.
So the answer is to make up the solution more frequently.
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© Roy C. Ellis 2002